Isolation of the canine inhibin βB subunit gene and characterization of signalling mediated by canine inhibin βB.
BMP pathway
TGF-β family
activin B
activin/TGF-β pathway
dog
Journal
Cell biochemistry and function
ISSN: 1099-0844
Titre abrégé: Cell Biochem Funct
Pays: England
ID NLM: 8305874
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
revised:
11
07
2021
received:
21
05
2021
accepted:
13
07
2021
pubmed:
13
8
2021
medline:
28
12
2021
entrez:
12
8
2021
Statut:
ppublish
Résumé
Activin B, a homodimer of the inhibin βB subunit, acts as a regulator of gonadal function and as an adipokine. To clarify the role of activin B in dogs, we characterized the canine inhibin βB gene and signalling pathways regulated by the canine inhibin βB. Using 5'- and 3'-rapid amplification of cDNA end (RACE) and RT-PCR on RNA isolated from the ovary of dogs, we identified short and long forms of the inhibin βB gene. Immunoreactive inhibin βB molecules were detected at ~25 and ~14 kDa under nonreducing and reducing conditions, respectively, in culture supernatants from HEK293 cells transfected with a plasmid containing the long form of the inhibin βB gene, indicating activin B production and secretion. Similar to human and murine activin B, the canine activin B-stimulated transcriptions of reporter genes, CAGA-luc and Hepcidin-luc, regulated by the canonical activin/transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) pathway, respectively. Activin B-induced CAGA-luc transcription was not detected in ALK7-deficient MDCK canine-derived cells; however, the forced expression of ALK7 resulted in the activin B-dependent expression in MDCK cells. Unexpectedly, the activin B-induced activation of the BMP pathway was partially blocked by the inhibition of endogenous activin/TGF-β receptor activity. The present study identified an experimentally isolated long form of the canine inhibin βB gene producing activin B that transactivates BMP- and activin/TGF-β-regulated gene expression.
Substances chimiques
Inhibin-beta Subunits
93443-12-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
970-982Subventions
Organisme : Japan Society for the Promotion of Science
ID : KAKENHI (18K05982)
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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