CircFOXM1 silencing represses cell proliferation, migration and invasion by regulating miR-515-5p/ADAM10 axis in prostate cancer.
ADAM10 Protein
/ metabolism
Amyloid Precursor Protein Secretases
/ metabolism
Animals
Cell Line, Tumor
Cell Movement
/ physiology
Cell Proliferation
/ physiology
Forkhead Box Protein M1
/ metabolism
Gene Knockdown Techniques
Humans
Male
Membrane Proteins
/ metabolism
Mice
Mice, Nude
MicroRNAs
/ metabolism
Prostatic Neoplasms
/ pathology
RNA, Circular
/ metabolism
Survival Analysis
Up-Regulation
Journal
Anti-cancer drugs
ISSN: 1473-5741
Titre abrégé: Anticancer Drugs
Pays: England
ID NLM: 9100823
Informations de publication
Date de publication:
01 01 2022
01 01 2022
Historique:
pubmed:
14
8
2021
medline:
5
3
2022
entrez:
13
8
2021
Statut:
ppublish
Résumé
Circular FOXM1 (circFOXM1) has been demonstrated to participate in the initiation and development of cancers, including prostate cancer (PCa). However, there is no relevant information on the regulation of PCa by circFOXM1. The RNA level of circFOXM1 was detected by qRT-PCR in PCa tissues and cells. The protein expression was performed by western blot and immunohistochemistry assay. Cell proliferation was examined by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide, colony formation and flow cytometry assays. The abilities of cell migration and invasion were determined by transwell assay. The relationship between circFOXM1 and miR-515-5p or ADAM10 was predicted by starBaseV2.0 online database, and identified by dual-luciferase reporter assay or RNA pull-down assay. The effects of circFOXM1 silencing and ADAM10 knockdown on PCa growth in vivo were evaluated by in-vivo tumor formation assay. As a result, we found that circFOXM1 and ADAM10 expression were upregulated in PCa tissues and cells. Functional analysis showed that circFOXM1 silencing repressed proliferation, migration and invasion, and induced cell cycle arrest, whereas these effects were partly reversed by miR-515-5p inhibitor. Additionally, circFOXM1 directly sponged miR-515-5p, and miR-515-5p bound to ADAM10. ADAM10 absence also repressed PCa process. Furthermore, in-vivo tumor formation assay revealed that both circFOXM1 silencing and ADAM10 knockdown repressed tumor growth in vivo. Thus, we came a conclusion that circFOXM1 contributed to PCa progression via regulating miR-515-5p/ADAM10 axis. These results may provide a theoretical basis for further studying the progression of PCa.
Identifiants
pubmed: 34387599
doi: 10.1097/CAD.0000000000001183
pii: 00001813-202201000-00075
doi:
Substances chimiques
Forkhead Box Protein M1
0
MIRN515 microRNA, human
0
Membrane Proteins
0
MicroRNAs
0
RNA, Circular
0
Amyloid Precursor Protein Secretases
EC 3.4.-
ADAM10 Protein
EC 3.4.24.81
ADAM10 protein, human
EC 3.4.24.81
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e573-e583Informations de copyright
Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.
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