Characterization of the virome associated with Haemagogus mosquitoes in Trinidad, West Indies.
Alphavirus Infections
/ epidemiology
Animals
Base Sequence
Birds
Culicidae
/ microbiology
Disease Outbreaks
Disease Reservoirs
/ virology
Geography, Medical
Host Specificity
Insect Vectors
/ virology
Phylogeny
Proteobacteria
/ genetics
RNA Viruses
/ classification
RNA, Bacterial
/ genetics
RNA, Viral
/ genetics
Rodentia
Togaviridae
/ genetics
Trinidad and Tobago
/ epidemiology
Virome
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 08 2021
16 08 2021
Historique:
received:
31
03
2021
accepted:
28
07
2021
entrez:
17
8
2021
pubmed:
18
8
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Currently, there are increasing concerns about the possibility of a new epidemic due to emerging reports of Mayaro virus (MAYV) fever outbreaks in areas of South and Central America. Haemagogus mosquitoes, the primary sylvan vectors of MAYV are poorly characterized and a better understanding of the mosquito's viral transmission dynamics and interactions with MAYV and other microorganisms would be important in devising effective control strategies. In this study, a metatranscriptomic based approach was utilized to determine the prevalence of RNA viruses in field-caught mosquitoes morphologically identified as Haemagogus janthinomys from twelve (12) forest locations in Trinidad, West Indies. Known insect specific viruses including the Phasi Charoen-like and Humaiata-Tubiacanga virus dominated the virome of the mosquitoes throughout sampling locations while other viruses such as the avian leukosis virus, MAYV and several unclassified viruses had a narrower distribution. Additionally, assembled contigs from the Ecclesville location suggests the presence of a unique uncharacterized picorna-like virus. Mapping of RNA sequencing reads to reference mitochondrial sequences of potential feeding host animals showed hits against avian and rodent sequences, which putatively adds to the growing body of evidence of a potentially wide feeding host-range for the Haemagogus mosquito vector.
Identifiants
pubmed: 34400676
doi: 10.1038/s41598-021-95842-6
pii: 10.1038/s41598-021-95842-6
pmc: PMC8368243
doi:
Substances chimiques
RNA, Bacterial
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16584Informations de copyright
© 2021. The Author(s).
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