Primary cell culture of canine corneal endothelial cells.
canine
cell culture
corneal endothelial cell
primary cell
Journal
Veterinary ophthalmology
ISSN: 1463-5224
Titre abrégé: Vet Ophthalmol
Pays: England
ID NLM: 100887377
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
14
07
2021
received:
01
03
2021
accepted:
27
07
2021
pubmed:
18
8
2021
medline:
9
11
2021
entrez:
17
8
2021
Statut:
ppublish
Résumé
To establish a primary cell culture and clarify the characteristics of canine corneal endothelial cells in vitro. The eyes were enucleated from dogs that were euthanized for reasons unrelated to this study. Enucleated canine eyes were dissected, and the intact corneas were isolated from the globes. Using enzymes, the corneal endothelial cells were dispersed from the cornea. The obtained canine corneal endothelial cells were cultured in a cell culture dish. Cultured corneal endothelial cells were morphologically evaluated using phase-contrast microscopy. Immunohistochemical analysis of the cultured cells, particularly of the corneal endothelial cell marker, zonula occludens-1 (ZO-1), Na Canine primary cultured corneal endothelial cells showed morphologically small, cobblestone-like structures. The isolated cells had proliferative ability in vitro and demonstrated positive expression of the corneal endothelial cell markers, ZO-1, Na This study demonstrated the successful culture of canine corneal endothelial cells. This might enhance the understanding of corneal endothelial cell characteristics in dogs.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
447-454Subventions
Organisme : Kitasato University Research Grant for Young Researchers
ID : 2228-3244
Organisme : Japan Society for the Promotion of Science
ID : JP20K15666
Informations de copyright
© 2021 American College of Veterinary Ophthalmologists.
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