Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan.
Anaplasma marginale
Layyah district
PCR
Phylogenetic analysis
Sheep
Theileria ovis
Journal
Tropical animal health and production
ISSN: 1573-7438
Titre abrégé: Trop Anim Health Prod
Pays: United States
ID NLM: 1277355
Informations de publication
Date de publication:
17 Aug 2021
17 Aug 2021
Historique:
received:
09
05
2021
accepted:
23
07
2021
entrez:
17
8
2021
pubmed:
18
8
2021
medline:
20
8
2021
Statut:
epublish
Résumé
Theileria ovis and Anaplasma marginale are intracellular pathogens affecting a wide range of animals, causing huge economic losses worldwide. The present study reports the molecular evidence of Theileria ovis and Anaplasma marginale in sheep blood samples (N = 218) collected from Layyah district in Punjab (Pakistan), where economy heavily relies on livestock. A 520 base pair fragment specific for 18S ribosomal RNA gene of Theileria ovis was PCR amplified in 23/218 (10.6%) sheep blood samples, while for Anaplasma marginale, a 265 base pair fragment specific for msp1b gene was generated in 15/218 (6.9%) sheep blood samples. Two blood samples were found co-infected (0.9%) with both parasites. Amplified PCR products of both parasites were confirmed by DNA sequencing and submitted to GenBank. Prevalence of both Theileria ovis (p = 0.3) and Anaplasma marginale (p = 0.4) varied non-significantly among the investigated sheep breeds. Tick burden on dogs present with sheep herds was found associated with Theileria ovis infection in sheep (p = 0.05). It was observed that lambs (p = 0.009), sheep in small herds (p = 0.04), and tick burden on dogs present with sheep herds (p = 0.01) were associated with Anaplasma marginale infection in sheep during the present study. In conclusion, we are reporting a higher prevalence of Theileria ovis than Anaplasma marginale in blood samples of sheep collected from Layyah district. Tick-infested dogs were found to be risk factors for the transmission of both pathogens in sheep, and tick control strategies should be extended to dogs associated with sheep herds in this area.
Identifiants
pubmed: 34402970
doi: 10.1007/s11250-021-02870-5
pii: 10.1007/s11250-021-02870-5
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
439Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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