Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
20 09 2021
20 09 2021
Historique:
accepted:
08
08
2021
revised:
01
08
2021
received:
27
06
2020
pubmed:
18
8
2021
medline:
9
11
2021
entrez:
17
8
2021
Statut:
ppublish
Résumé
Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present orthogonal strategies to produce densely modified RCA products and DNFs. We provide methods to selectively modify the DNA component and/or the protein cargo of these materials, thereby greatly expanding the range of chemical functionalities available to these systems. We have used our methodology to construct DNFs bearing multiple surface aptamers and peptides capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating their potential for diagnostic and therapeutic applications.
Identifiants
pubmed: 34403467
pii: 6353807
doi: 10.1093/nar/gkab720
pmc: PMC8450075
doi:
Substances chimiques
Aptamers, Peptide
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9042-9052Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/J001694/2
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/ R008655/1
Pays : United Kingdom
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
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