Comparison of four commercial, automated antigen tests to detect SARS-CoV-2 variants of concern.


Journal

Medical microbiology and immunology
ISSN: 1432-1831
Titre abrégé: Med Microbiol Immunol
Pays: Germany
ID NLM: 0314524

Informations de publication

Date de publication:
Dec 2021
Historique:
received: 08 08 2021
accepted: 13 08 2021
pubmed: 21 8 2021
medline: 28 10 2021
entrez: 20 8 2021
Statut: ppublish

Résumé

A versatile portfolio of diagnostic tests is essential for the containment of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) pandemic. Besides nucleic acid-based test systems and point-of-care (POCT) antigen (Ag) tests, quantitative, laboratory-based nucleocapsid Ag tests for SARS-CoV-2 have recently been launched. Here, we evaluated four commercial Ag tests on automated platforms and one POCT to detect SARS-CoV-2. We evaluated PCR-positive (n = 107) and PCR-negative (n = 303) respiratory swabs from asymptomatic and symptomatic patients at the end of the second pandemic wave in Germany (February-March 2021) as well as clinical isolates EU1 (B.1.117), variant of concern (VOC) Alpha (B.1.1.7) or Beta (B.1.351), which had been expanded in a biosafety level 3 laboratory. The specificities of automated SARS-CoV-2 Ag tests ranged between 97.0 and 99.7% (Lumipulse G SARS-CoV-2 Ag (Fujirebio): 97.03%, Elecsys SARS-CoV-2 Ag (Roche Diagnostics): 97.69%; LIAISON

Identifiants

pubmed: 34415422
doi: 10.1007/s00430-021-00719-0
pii: 10.1007/s00430-021-00719-0
pmc: PMC8377707
doi:

Substances chimiques

Antigens, Viral 0

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

263-275

Subventions

Organisme : Bayerisches Staatsministerium für Bildung und Kultus, Wissenschaft und Kunst
ID : FORCOVID
Organisme : Bayerisches Staatsministerium für Bildung und Kultus, Wissenschaft und Kunst
ID : BayVOC

Informations de copyright

© 2021. The Author(s).

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Auteurs

Andreas Osterman (A)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.

Maximilian Iglhaut (M)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.

Andreas Lehner (A)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.

Patricia Späth (P)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.

Marcel Stern (M)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.

Hanna Autenrieth (H)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.

Maximilian Muenchhoff (M)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
German Center for Infection Research (DZIF), Partner Site, Munich, Germany.
COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany.

Alexander Graf (A)

Laboratory for Functional Genome Analysis, Gene Center, LMU München, Munich, Germany.

Stefan Krebs (S)

Laboratory for Functional Genome Analysis, Gene Center, LMU München, Munich, Germany.

Helmut Blum (H)

Laboratory for Functional Genome Analysis, Gene Center, LMU München, Munich, Germany.

Armin Baiker (A)

Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.

Natascha Grzimek-Koschewa (N)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
German Center for Infection Research (DZIF), Partner Site, Munich, Germany.

Ulrike Protzer (U)

German Center for Infection Research (DZIF), Partner Site, Munich, Germany.
Institute of Virology, Technical University of Munich/Helmholtz Zentrum München, Munich, Germany.

Lars Kaderali (L)

Institute of Bioinformatics, University Medicine Greifswald, Greifswald, Germany.

Hanna-Mari Baldauf (HM)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany. baldauf@mvp.lmu.de.
Max Von Pettenkofer Institute, Virology, National Reference Center for Retroviruses, LMU München, Feodor-Lynen-Str. 23, 81377, Munich, Germany. baldauf@mvp.lmu.de.

Oliver T Keppler (OT)

Max Von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany. keppler@mvp.lmu.de.
German Center for Infection Research (DZIF), Partner Site, Munich, Germany. keppler@mvp.lmu.de.
COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany. keppler@mvp.lmu.de.
Max Von Pettenkofer Institute, Virology, National Reference Center for Retroviruses, LMU München, Pettenkoferstr. 9a, 80336, Munich, Germany. keppler@mvp.lmu.de.

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