Measurement of Cell Death in Mammalian Cells.
apoptosis
cytotoxicity
necrosis
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
entrez:
20
8
2021
pubmed:
21
8
2021
medline:
25
8
2021
Statut:
ppublish
Résumé
Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways of cell death; (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity; (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis and apoptosis; (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis; (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology; and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Measurement of plasma membrane integrity and viability using LDH release Basic Protocol 2: Measurement of necrosis and apoptosis using flow cytometry Basic Protocol 3: Determination of nuclear morphology and membrane integrity Alternate Protocol 1: Assessment of nuclear morphology and membrane integrity using DAPI and PI Alternate Protocol 2: Assessment of nuclear morphology using multi-well plates Basic Protocol 4: Measurement of time-dependent toxicity using cell death markers.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e210Subventions
Organisme : Department of Defense Spinal Cord Injury Research Program
ID : SC180122
Organisme : Biomedical Laboratory Research and Development Program of the Department of Veterans Affairs
ID : BX 005218
Organisme : Biomedical Laboratory Research and Development Program of the Department of Veterans Affairs
ID : BX 004868
Organisme : Biomedical Laboratory Research and Development Program of the Department of Veterans Affairs
ID : RX 003224
Organisme : Biomedical Laboratory Research and Development Program of the Department of Veterans Affairs
ID : BX 000851
Informations de copyright
© 2021 Wiley Periodicals LLC.
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