Ketoconazole resistant Candida albicans is sensitive to a wireless electroceutical wound care dressing.
Antibiotic resistance
Antibiotic textile
Biofilm
Electric field
Wound
Yeast infection
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
23
03
2021
revised:
27
07
2021
accepted:
31
07
2021
pubmed:
23
8
2021
medline:
1
2
2022
entrez:
22
8
2021
Statut:
ppublish
Résumé
Wireless electroceutical dressing (WED) fabric kills bacteria and disrupts bacterial biofilm. This work tested, comparing with standard of care topical antibiotic ketoconazole, whether the weak electric field generated by WED is effective to manage infection caused by ketoconazole-resistant yeast Candida albicans. WED inhibited Candida albicans biofilm formation and planktonic growth. Unlike ketoconazole, WED inhibited yeast to hyphal transition and downregulated EAP1 curbing cell attachment. In response to WED-dependent down-regulation of biofilm-forming BRG1 and ROB1, BCR1 expression was markedly induced in what seems to be a futile compensatory response. WED induced NRG1 and TUP1, negative regulators of filamentation; it down-regulated EFG1, a positive regulator of hyphal pathway. Consistent with the anti-hyphal properties of WED, the expression of ALS3 and HWP1 were diminished. Ketoconazole failed to reproduce the effects of WED on NRG1, TUP1 and EFG1. WED blunted efflux pump activity; this effect was in direct contrast to that of ketoconazole. WED exposure compromised cellular metabolism. In the presence of ketoconazole, the effect was synergistic. Unlike ketoconazole, WED caused membrane depolarization, changes in cell wall composition and loss of membrane integrity. This work presents first evidence that weak electric field is useful in managing pathogens which are otherwise known to be antibiotic resistant.
Identifiants
pubmed: 34419917
pii: S1567-5394(21)00184-5
doi: 10.1016/j.bioelechem.2021.107921
pmc: PMC8788813
mid: NIHMS1734790
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107921Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK125835
Pays : United States
Organisme : NINR NIH HHS
ID : R01 NR015676
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest CKS, in his capacity as a co-developer of the technology, has minor commercial interest in Vomaris Inc. He is also a paid consultant for the company.
Références
Synth Syst Biotechnol. 2016 Oct 25;1(3):158-168
pubmed: 29062940
Handb Exp Pharmacol. 2012;(211):135-52
pubmed: 23090600
J Infect Dis. 2017 Aug 15;216(suppl_3):S445-S451
pubmed: 28911043
Nucleic Acids Res. 2012 Aug;40(15):e115
pubmed: 22730293
Biomed Res Int. 2013;2013:204237
pubmed: 23878798
Mycoses. 2020 Jun;63(6):535-542
pubmed: 32077536
Biochim Biophys Acta. 1993 Nov 7;1179(2):224-8
pubmed: 8218365
J Bacteriol. 1971 Jan;105(1):1-5
pubmed: 5541005
Antimicrob Agents Chemother. 2016 Dec 27;61(1):
pubmed: 27821447
Antimicrob Agents Chemother. 1976 Nov;10(5):856-60
pubmed: 1034467
Infect Dis (Lond). 2018 Mar;50(3):214-219
pubmed: 28988525
Front Microbiol. 2012 Aug 08;3:286
pubmed: 23024638
ACS Appl Mater Interfaces. 2019 Nov 6;11(44):40997-41008
pubmed: 31603300
PLoS Pathog. 2019 May 22;15(5):e1007787
pubmed: 31116789
Front Microbiol. 2020 Jan 09;10:2993
pubmed: 31993032
PLoS One. 2015 Mar 24;10(3):e0119531
pubmed: 25803639
Carbohydr Polym. 2013 Apr 15;94(1):364-71
pubmed: 23544550
Anal Biochem. 2009 Nov 1;394(1):87-91
pubmed: 19577533
BMC Microbiol. 2014 Dec 05;14:303
pubmed: 25476750
Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13594-9
pubmed: 23904484
Microbiol Mol Biol Rev. 2007 Jun;71(2):348-76
pubmed: 17554048
Nat Microbiol. 2018 Aug;3(8):920-931
pubmed: 30038307
Microb Cell. 2019 Jul 01;6(7):299-306
pubmed: 31294042
iScience. 2020 Feb 21;23(2):100827
pubmed: 32058950
BMC Microbiol. 2014 Jul 05;14:182
pubmed: 24996549
Am Fam Physician. 2014 Apr 1;89(7):569-73
pubmed: 24695603
Front Microbiol. 2016 Feb 02;7:64
pubmed: 26870022
Mol Cell Biol. 2000 Oct;20(20):7654-61
pubmed: 11003661
Antimicrob Agents Chemother. 2019 Apr 25;63(5):
pubmed: 30782996
Biochim Biophys Acta Biomembr. 2017 Oct;1859(10):1974-1985
pubmed: 28669766
Ann Surg. 2019 Apr;269(4):756-766
pubmed: 29099398
Cell Microbiol. 2012 Sep;14(9):1319-35
pubmed: 22587014
PLoS One. 2014 Mar 03;9(3):e89239
pubmed: 24595050
J Microbiol Methods. 2009 Aug;78(2):208-12
pubmed: 19501122
EMBO J. 2001 Sep 3;20(17):4753-61
pubmed: 11532939
Int J Bacteriol. 2016;2016:9727810
pubmed: 27073807
Front Microbiol. 2017 Jan 12;7:2173
pubmed: 28127295
Antimicrob Agents Chemother. 2016 Mar 25;60(4):2326-35
pubmed: 26833156
Bioelectrochemistry. 2019 Aug;128:148-154
pubmed: 31003053
Front Microbiol. 2012 Apr 05;3:133
pubmed: 22493595
J Med Microbiol. 2009 Jun;58(Pt 6):779-790
pubmed: 19429755
FEMS Yeast Res. 2006 Jan;6(1):14-29
pubmed: 16423067
PLoS One. 2012;7(3):e33705
pubmed: 22479431
Int J Clin Exp Med. 2015 Oct 15;8(10):19188-99
pubmed: 26770555
Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10570-4
pubmed: 7479842
mBio. 2015 Jul 28;6(4):e00986
pubmed: 26220968
Materials (Basel). 2016 Jun 21;9(6):
pubmed: 28773619
J Mycol Med. 2017 Dec;27(4):469-475
pubmed: 28754462
Eukaryot Cell. 2013 Feb;12(2):254-64
pubmed: 23243062
PLoS Genet. 2017 Aug 9;13(8):e1006948
pubmed: 28793308
Future Microbiol. 2018 Apr;13:535-546
pubmed: 29227694
Mol Ther. 2018 Sep 5;26(9):2178-2188
pubmed: 29802017
Microbiol Spectr. 2015 Jun;3(3):
pubmed: 26185083
Microbiology (Reading). 2008 Nov;154(Pt 11):3266-3280
pubmed: 18957581
J Wound Care. 2011 Jan;20(1):40-7
pubmed: 21278640
Sci Rep. 2017 Jul 18;7(1):5692
pubmed: 28720834
J Fungi (Basel). 2018 Jun 04;4(2):
pubmed: 29866990
Microbiol Immunol. 1997;41(5):395-402
pubmed: 9194038
J Bacteriol. 1996 Apr;178(8):2416-9
pubmed: 8636047
Antimicrob Agents Chemother. 2018 Mar 27;62(4):
pubmed: 29358288
FEMS Microbiol Rev. 2015 Nov;39(6):797-811
pubmed: 26253139
Antibiotics (Basel). 2020 Jun 09;9(6):
pubmed: 32526921
Infect Immun. 2011 Dec;79(12):4902-11
pubmed: 21930756
Infect Drug Resist. 2019 Jul 08;12:1985-2001
pubmed: 31372006
Antimicrob Agents Chemother. 2001 Jun;45(6):1660-70
pubmed: 11353609
Eukaryot Cell. 2010 Sep;9(9):1363-73
pubmed: 20656914
Bioelectromagnetics. 2017 Feb;38(2):95-108
pubmed: 27783424
Annu Rev Microbiol. 2015;69:71-92
pubmed: 26488273
J Clin Microbiol. 2010 Jun;48(6):2097-102
pubmed: 20410345
Eukaryot Cell. 2003 Dec;2(6):1266-73
pubmed: 14665461
Microbiol Spectr. 2016 Jun;4(3):
pubmed: 27337476
J Wound Care. 2015 Jan;24(1):23-33
pubmed: 25543820
PLoS Pathog. 2017 May 22;13(5):e1006403
pubmed: 28542528
Trends Microbiol. 2014 Dec;22(12):707-14
pubmed: 25262420
G3 (Bethesda). 2018 Feb 2;8(2):643-652
pubmed: 29233914
Yeast. 2010 Feb;27(2):77-87
pubmed: 19908200
Front Microbiol. 2020 Mar 17;11:296
pubmed: 32256459
Burns. 2019 Aug;45(5):1164-1171
pubmed: 30686692
Nature. 2016 Apr 7;532(7597):64-8
pubmed: 27027296
Trends Microbiol. 2014 Nov;22(11):614-22
pubmed: 25088819
Eukaryot Cell. 2011 Aug;10(8):1004-12
pubmed: 21642508
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712