TLR5 signalling is hyper-responsive in porcine cystic fibrosis airways epithelium.
Journal
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
ISSN: 1873-5010
Titre abrégé: J Cyst Fibros
Pays: Netherlands
ID NLM: 101128966
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
09
02
2021
revised:
07
06
2021
accepted:
04
08
2021
pubmed:
24
8
2021
medline:
14
4
2022
entrez:
23
8
2021
Statut:
ppublish
Résumé
Excessive lung inflammation and airway epithelium damage are hallmarks of cystic fibrosis (CF) disease. It is unclear whether lung inflammation is related to an intrinsic defect in the immune response or to chronic infection. We aimed to determine whether TLR5-mediated response is defective in the CF airway epithelium. We used a newborn CF pig model to study intrinsic alterations in CF airway epithelium innate immune response. Airway epithelial cells (AECs) were stimulated with flagellin or lipopolysaccharide to determine responses specific for TLR5 and TLR4, respectively. We observed a significant increase in cytokine secretion when CF AECs were stimulated with flagellin compared to wild type (WT) AECs. These results were recapitulated when AECs were treated with an inhibitor of CFTR channel activity. We show that TLR5-signalling is altered in CF lung epithelium at birth. Modulation of TLR5 signalling could contribute to better control the excessive inflammatory response observed in CF lungs.
Identifiants
pubmed: 34420900
pii: S1569-1993(21)01343-6
doi: 10.1016/j.jcf.2021.08.002
pii:
doi:
Substances chimiques
Toll-Like Receptor 5
0
Cystic Fibrosis Transmembrane Conductance Regulator
126880-72-6
Flagellin
12777-81-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e117-e121Informations de copyright
Copyright © 2021. Published by Elsevier B.V.