Accessible Platform for High-Throughput COVID-19 Molecular Diagnostics and Genome Sequencing Using a Repurposed 3D Printer for RNA Extraction.

COVID-19 Humans Pandemics Pathology, Molecular RNA, Viral / genetics SARS-CoV-2
3D printer COVID-19 RNA extraction and purification RT-PCR SARS-CoV-2 genome sequencing molecular diagnostics

Journal

ACS biomaterials science & engineering
ISSN: 2373-9878
Titre abrégé: ACS Biomater Sci Eng
Pays: United States
ID NLM: 101654670

Informations de publication

Date de publication:
13 09 2021
Historique:
pubmed: 27 8 2021
medline: 18 9 2021
entrez: 26 8 2021
Statut: ppublish

Résumé

The COVID-19 pandemic has exposed the dependence of diagnostic laboratories on a handful of large corporations with market monopolies on the worldwide supply of reagents, consumables, and hardware for molecular diagnostics. Global shortages of key consumables for RT-qPCR detection of SARS-CoV-2 RNA have impaired the ability to run essential, routine diagnostic services. Here, we describe a workflow for rapid detection of SARS-CoV-2 RNA in upper respiratory samples including nasal swabs and saliva, utilizing low-cost equipment and readily accessible reagents. Using repurposed

Identifiants

DOI: 10.1021/acsbiomaterials.1c00775 PMID: 34437802
pubmed: 34437802
doi: 10.1021/acsbiomaterials.1c00775
doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4669-4676

Auteurs

Koen Vandelannoote (K)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Andrew H Buultjens (AH)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
ORCID: 0000-0002-5984-1328

Lucy Li (L)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Liam K Sharkey (LK)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Marion Herisse (M)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Sacha J Pidot (SJ)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
ORCID: 0000-0003-1202-6614

Tuyet Hoang (T)

Microbiological Diagnostic Unit Public Health Laboratory, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Benjamin P Howden (BP)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
Microbiological Diagnostic Unit Public Health Laboratory, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
Department of Infectious Diseases, Austin Health, 145 Studley Rd, Heidelberg, Victoria 3084, Australia.

Ian R Monk (IR)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Torsten Seemann (T)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
Microbiological Diagnostic Unit Public Health Laboratory, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.

Jean Y H Lee (JYH)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
Department of Infectious Diseases, Monash Health, 246 Clayton Rd, Clayton, Victoria 3168, Australia.

Timothy P Stinear (TP)

Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, 792 Elizabeth St, Melbourne, Victoria 3000, Australia.
ORCID: 0000-0003-0150-123X

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Classifications MeSH

questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Accessible Platform for High-Throughput...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Accessible Platform for High-Throughput...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Épidémies de maladies › Épidémies › Pandémies › Accessible Platform for High-Throughput...
questionsmedicales.fr › Professions de santé › Médecine › Anatomopathologie › Anatomopathologie moléculaire › Accessible Platform for High-Throughput...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Accessible Platform for High-Throughput...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Accessible Platform for High-Throughput...