Amnion signals are essential for mesoderm formation in primates.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 08 2021
26 08 2021
Historique:
received:
12
11
2020
accepted:
22
07
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
23
9
2021
Statut:
epublish
Résumé
Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1, a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 results in non-human primate embryos which do not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos display a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirm a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.
Identifiants
pubmed: 34446705
doi: 10.1038/s41467-021-25186-2
pii: 10.1038/s41467-021-25186-2
pmc: PMC8390679
doi:
Substances chimiques
Bone Morphogenetic Protein 4
0
LIM-Homeodomain Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
5126Subventions
Organisme : NICHD NIH HHS
ID : R21 HD100931
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS113518
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Informations de copyright
© 2021. The Author(s).
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