Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate.

Adenosine Triphosphatases / chemistry Allosteric Regulation Binding Sites Cloning, Molecular Cryoelectron Microscopy Deuterium Exchange Measurement Enteropathogenic Escherichia coli / genetics Escherichia coli Proteins / chemistry Flagella / genetics Gene Expression Gene Expression Regulation, Bacterial Genetic Vectors / chemistry Kinetics Mass Spectrometry Models, Molecular Molecular Chaperones / chemistry Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs Protein Subunits / chemistry Recombinant Proteins / chemistry SEC Translocation Channels / chemistry Substrate Specificity Type III Secretion Systems / genetics
EPEC SctV-C structure export apparatus protein dynamics type III secretion

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
15 10 2021
Historique:
received: 10 06 2021
revised: 30 07 2021
accepted: 02 08 2021
pubmed: 30 8 2021
medline: 25 11 2021
entrez: 29 8 2021
Statut: ppublish

Résumé

Type III protein secretion is widespread in Gram-negative pathogens. It comprises the injectisome with a surface-exposed needle and an inner membrane translocase. The translocase contains the SctRSTU export channel enveloped by the export gate subunit SctV that binds chaperone/exported clients and forms a putative ante-chamber. We probed the assembly, function, structure and dynamics of SctV from enteropathogenic E. coli (EPEC). In both EPEC and E. coli lab strains, SctV forms peripheral oligomeric clusters that are detergent-extracted as homo-nonamers. Membrane-embedded SctV

Identifiants

DOI: 10.1016/j.jmb.2021.167188 PMID: 34454944
pubmed: 34454944
pii: S0022-2836(21)00421-6
doi: 10.1016/j.jmb.2021.167188
pii:
doi:

Substances chimiques

Escherichia coli Proteins 0
Molecular Chaperones 0
Protein Subunits 0
Recombinant Proteins 0
SEC Translocation Channels 0
Type III Secretion Systems 0
Adenosine Triphosphatases EC 3.6.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

167188

Informations de copyright

Crown Copyright © 2021. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Competing interests The authors declare they have no competing financial interests or other conflicts of interest

Auteurs

Biao Yuan (B)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium; Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany; University Medical Center Hamburg-Eppendorf (UKE), Institute for Structural and Systems Biology, Notkestrasse 85, D-22607 Hamburg, Germany; German Electron Synchrotron Centre (DESY), Notkestrasse 85, D-22607 Hamburg, Germany. Electronic address: biao.yuan@cssb-hamburg.de.

Athina G Portaliou (AG)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium. Electronic address: athina.portaliou@kuleuven.be.

Rinky Parakra (R)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium. Electronic address: rinky.parakra@kuleuven.be.

Jochem H Smit (JH)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium; Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands. Electronic address: jochem.smit@kuleuven.be.

Jiri Wald (J)

Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany; University Medical Center Hamburg-Eppendorf (UKE), Institute for Structural and Systems Biology, Notkestrasse 85, D-22607 Hamburg, Germany; German Electron Synchrotron Centre (DESY), Notkestrasse 85, D-22607 Hamburg, Germany. Electronic address: jiri.wald@cssb-hamburg.de.

Yichen Li (Y)

Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands. Electronic address: yichen.li@rug.nl.

Bindu Srinivasu (B)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium. Electronic address: bindu.srinivasu@kuleuven.be.

Maria S Loos (MS)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium.

Harveer Singh Dhupar (HS)

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Life Sciences Institute, University of British Columbia, Vancouver, Canada.

Dirk Fahrenkamp (D)

Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany; University Medical Center Hamburg-Eppendorf (UKE), Institute for Structural and Systems Biology, Notkestrasse 85, D-22607 Hamburg, Germany; German Electron Synchrotron Centre (DESY), Notkestrasse 85, D-22607 Hamburg, Germany. Electronic address: dirk.fahrenkamp@cssb-hamburg.de.

Charalampos G Kalodimos (CG)

Department of Structural Biology, St. Jude Children's Research Hospital, 263 Danny Thomas Place, Memphis, TN 38105, United States. Electronic address: babis.kalodimos@stjude.org.

Franck Duong van Hoa (F)

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Life Sciences Institute, University of British Columbia, Vancouver, Canada. Electronic address: fduong@mail.ubc.ca.

Thorben Cordes (T)

Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands; Physical and Synthetic Biology, Faculty of Biology, Ludwig Maximilians-Universität München, Großhadernerstr. 2-4, 82152 Planegg-Martinsried, Germany. Electronic address: cordes@bio.lmu.de.

Spyridoula Karamanou (S)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium. Electronic address: lily.karamanou@kuleuven.be.

Thomas C Marlovits (TC)

Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany; University Medical Center Hamburg-Eppendorf (UKE), Institute for Structural and Systems Biology, Notkestrasse 85, D-22607 Hamburg, Germany; German Electron Synchrotron Centre (DESY), Notkestrasse 85, D-22607 Hamburg, Germany. Electronic address: thomas.marlovits@cssb-hamburg.de.

Anastassios Economou (A)

KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, B-3000 Leuven, Belgium. Electronic address: tassos.economou@kuleuven.be.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Acid anhydride hydrolases › Adenosine triphosphatases › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Régulation allostérique › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Structural Dynamics of the Functional...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Structural Dynamics of the Functional...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Structural Dynamics of the Functional...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Mesure d'échange de deutérium › Structural Dynamics of the Functional...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli entéropathogène › Structural Dynamics of the Functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Structural Dynamics of the Functional...
questionsmedicales.fr › Cellules › Structures cellulaires › Prolongements cytoplasmiques › Flagelles › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Structural Dynamics of the Functional...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Structural Dynamics of the Functional...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structural Dynamics of the Functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Structural Dynamics of the Functional...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Structure en hélice alpha › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Structure en brin bêta › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs et domaines d'intéraction protéique › Structural Dynamics of the Functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Sous-unités de protéines › Structural Dynamics of the Functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Structural Dynamics of the Functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux de translocation SEC › Structural Dynamics of the Functional...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Structural Dynamics of the Functional...
questionsmedicales.fr › Structures macromoléculaires › Complexes multiprotéiques › Systèmes de translocation des protéines › Systèmes bactériens de sécrétion › Systèmes de sécrétion de type III › Structural Dynamics of the Functional...