In Vitro and In Vivo Evaluation of Lacticaseibacillus rhamnosus GG and Bifidobacterium lactis Bb12 Against Avian Pathogenic Escherichia coli and Identification of Novel Probiotic-Derived Bioactive Peptides.
Animals
Escherichia coli
Bifidobacterium animalis
Chickens
Chromatography, Liquid
Tandem Mass Spectrometry
Escherichia coli Infections
/ microbiology
Probiotics
/ pharmacology
Poultry Diseases
/ drug therapy
Lacticaseibacillus rhamnosus
Anti-Bacterial Agents
/ pharmacology
Poultry
Peptides
/ pharmacology
APEC
Antibiotic alternatives
Chickens
L. rhamnosus GG
Peptides
Probiotics
Journal
Probiotics and antimicrobial proteins
ISSN: 1867-1314
Titre abrégé: Probiotics Antimicrob Proteins
Pays: United States
ID NLM: 101484100
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
accepted:
23
08
2021
pubmed:
31
8
2021
medline:
22
11
2022
entrez:
30
8
2021
Statut:
ppublish
Résumé
Avian pathogenic E. coli (APEC), an extra-intestinal pathogenic E. coli (ExPEC), causes colibacillosis in poultry and is also a potential foodborne zoonotic pathogen. Currently, APEC infections in poultry are controlled by antibiotic medication; however, the emergence of multi-drug-resistant APEC strains and increased restrictions on the use of antibiotics in food-producing animals necessitate the development of new antibiotic alternative therapies. Here, we tested the anti-APEC activity of multiple commensal and probiotic bacteria in an agar-well diffusion assay and identified Lacticaseibacillus rhamnosus GG and Bifidobacterium lactis Bb12 producing strong zone of inhibition against APEC. In co-culture assay, L. rhamnosus GG and B. lactis Bb12 completely inhibited the APEC growth by 24 h. Further investigation revealed that antibacterial product(s) in the culture supernatants of L. rhamnosus GG and B. lactis Bb12 were responsible for the anti-APEC activity. The analysis of culture supernatants using LC-MS/MS identified multiple novel bioactive peptides (VQAAQAGDTKPIEV, AFDNTDTSLDSTFKSA, VTDTSGKAGTTKISNV, and AESSDTNLVNAKAA) in addition to the production of lactic acid. The oral administration (10
Identifiants
pubmed: 34458959
doi: 10.1007/s12602-021-09840-1
pii: 10.1007/s12602-021-09840-1
doi:
Substances chimiques
Anti-Bacterial Agents
0
Peptides
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1012-1028Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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