The fungivorous amoeba Protostelium aurantium targets redox homeostasis and cell wall integrity during intracellular killing of Candida parapsilosis.
Candida
amoeba
phagocytosis
protostelium
yeasts
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
08
03
2021
received:
15
07
2019
accepted:
26
08
2021
pubmed:
31
8
2021
medline:
14
1
2022
entrez:
30
8
2021
Statut:
ppublish
Résumé
Predatory interactions among microbes are major evolutionary driving forces for biodiversity. The fungivorous amoeba Protostelium aurantium has a wide fungal food spectrum including foremost pathogenic members of the genus Candida. Here we show that upon phagocytic ingestion by the amoeba, Candida parapsilosis is confronted with an oxidative burst and undergoes lysis within minutes of processing in acidified phagolysosomes. On the fungal side, a functional genomic approach identified copper and redox homeostasis as primary targets of amoeba predation, with the highly expressed copper exporter gene CRP1 and the peroxiredoxin gene PRX1 contributing to survival when encountered with P. aurantium. The fungicidal activity was largely retained in intracellular vesicles of the amoebae. Following their isolation, the content of these vesicles induced immediate killing and lysis of C. parapsilosis in vitro. Proteomic analysis identified 56 vesicular proteins from P. aurantium. Although completely unknown proteins were dominant, many of them could be categorised as hydrolytic enzymes targeting the fungal cell wall, indicating that fungal cell wall structures are under selection pressure by predatory phagocytes in natural environments. TAKE AWAY: The amoeba Protostelium aurantium feeds on fungi, such as Candida parapsilosis. Ingested yeast cells are exposed to reactive oxygen species. A copper exporter and a peroxiredoxin contribute to fungal defence. Yeast cells undergo intracellular lysis. Lysis occurs via a cocktail of hydrolytic enzymes from intracellular vesicles.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13389Subventions
Organisme : Gazdaságfejlesztési és Innovációs Operatív Program
ID : GINOP-2.3.2-15-2016-00035
Organisme : Gazdaságfejlesztési és Innovációs Operatív Program
ID : GINOP-2.3.3-15-2016-00006
Organisme : Deutscher Akademischer Austausch Dienst
Organisme : European Social Fund ESF "Europe for Thuringia"
ID : 2016FGR0053
Organisme : National Research, Development and Innovation Office Hungary
ID : NKFIH K123952
Organisme : German Academic Exchange Service
Organisme : European Social Fund
Organisme : Research Foundation
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC 2051 - Project-ID 390713860
Organisme : Deutsche Forschungsgemeinschaft
ID : HI 1574/2-1
Organisme : Deutsche Forschungsgemeinschaft
ID : HI 1574/4-1
Organisme : Deutsche Forschungsgemeinschaft
ID : Hu 528/17-1
Organisme : Deutsche Forschungsgemeinschaft
ID : Hu 528/21-1
Organisme : Deutsche Forschungsgemeinschaft
ID : TRR 124 FungiNet
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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