Rational Design and Experimental Analysis of Short-Oligonucleotide Substrate Specificity for Targeting Bacterial Nucleases.


Journal

Journal of medicinal chemistry
ISSN: 1520-4804
Titre abrégé: J Med Chem
Pays: United States
ID NLM: 9716531

Informations de publication

Date de publication:
09 09 2021
Historique:
pubmed: 31 8 2021
medline: 15 12 2021
entrez: 30 8 2021
Statut: ppublish

Résumé

An undecamer oligonucleotide probe based on a pair of deoxythymidines flanked by several modified nucleotides is a specific and highly efficient biosensor for micrococcal nuclease (MNase), an endonuclease produced by

Identifiants

pubmed: 34460263
doi: 10.1021/acs.jmedchem.1c00884
pmc: PMC8436206
doi:

Substances chimiques

Bacterial Proteins 0
Oligonucleotides 0
Deoxyribonucleases EC 3.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

12855-12864

Références

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Auteurs

Tania Jiménez (T)

Somaprobes S.L, Mikeletegi Pasealekua, 83, 20009 Donostia, Gipuzkoa, Spain.

Juliana Botero (J)

Somaprobes S.L, Mikeletegi Pasealekua, 83, 20009 Donostia, Gipuzkoa, Spain.
Applied Chemistry Department, University of the Basque Country (UPV/EHU), 20018 San Sebastián, Spain.

Dorleta Otaegui (D)

Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), San Sebastian 20014, Spain.

Javier Calvo (J)

Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), San Sebastian 20014, Spain.

Frank J Hernandez (FJ)

Wallenberg Center for Molecular Medicine (WCMM), 58185 Linköping, Sweden.
Department of Physics, Chemistry and Biology, Linköping University, 58185 Linköping, Sweden.

Eider San Sebastian (E)

Applied Chemistry Department, University of the Basque Country (UPV/EHU), 20018 San Sebastián, Spain.

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Classifications MeSH