Genetic variations in AURORA cell cycle kinases are associated with glioblastoma multiforme.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
31 08 2021
Historique:
received: 17 06 2021
accepted: 18 08 2021
entrez: 1 9 2021
pubmed: 2 9 2021
medline: 9 11 2021
Statut: epublish

Résumé

Glioblastoma multiforme (GBM) is the most frequent type of primary astrocytomas. We examined the association between single nucleotide polymorphisms (SNPs) in Aurora kinase A (AURKA), Aurora kinase B (AURKB), Aurora kinase C (AURKC) and Polo-like kinase 1 (PLK1) mitotic checkpoint genes and GBM risk by qPCR genotyping. In silico analysis was performed to evaluate effects of polymorphic biological sequences on protein binding motifs. Chi-square and Fisher statistics revealed a significant difference in genotypes frequencies between GBM patients and controls for AURKB rs2289590 variant (p = 0.038). Association with decreased GBM risk was demonstrated for AURKB rs2289590 AC genotype (OR = 0.54; 95% CI = 0.33-0.88; p = 0.015). Furthermore, AURKC rs11084490 CG genotype was associated with lower GBM risk (OR = 0.57; 95% CI = 0.34-0.95; p = 0.031). Bioinformatic analysis of rs2289590 polymorphic region identified additional binding site for the Yin-Yang 1 (YY1) transcription factor in the presence of C allele. Our results indicated that rs2289590 in AURKB and rs11084490 in AURKC were associated with a reduced GBM risk. The present study was performed on a less numerous but ethnically homogeneous population. Hence, future investigations in larger and multiethnic groups are needed to strengthen these results.

Identifiants

pubmed: 34465813
doi: 10.1038/s41598-021-96935-y
pii: 10.1038/s41598-021-96935-y
pmc: PMC8408269
doi:

Substances chimiques

Biomarkers, Tumor 0
Cell Cycle Proteins 0
Proto-Oncogene Proteins 0
AURKA protein, human EC 2.7.11.1
AURKB protein, human EC 2.7.11.1
AURKC protein, human EC 2.7.11.1
Aurora Kinase A EC 2.7.11.1
Aurora Kinase B EC 2.7.11.1
Aurora Kinase C EC 2.7.11.1
Protein Serine-Threonine Kinases EC 2.7.11.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

17444

Subventions

Organisme : EPA
ID : EP-C-15-010
Pays : United States

Informations de copyright

© 2021. The Author(s).

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Auteurs

Aner Mesic (A)

Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000, Sarajevo, Bosnia and Herzegovina.

Marija Rogar (M)

Medical Centre for Molecular Biology, Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia.

Petra Hudler (P)

Medical Centre for Molecular Biology, Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia. petra.hudler@mf.uni-lj.si.

Nurija Bilalovic (N)

Clinical Pathology and Cytology, University Clinical Centre Sarajevo, Bolnička 25, 71000, Sarajevo, Bosnia and Herzegovina.

Izet Eminovic (I)

Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000, Sarajevo, Bosnia and Herzegovina.

Radovan Komel (R)

Medical Centre for Molecular Biology, Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia.

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Classifications MeSH