Functional analysis of the transcriptional activator XlnR of Penicillium oxalicum.
Penicillium
fungi
gene expression
genetic
molecular genetic
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
31
07
2021
received:
11
04
2021
accepted:
23
08
2021
pubmed:
2
9
2021
medline:
22
1
2022
entrez:
1
9
2021
Statut:
ppublish
Résumé
The aim of this article is to study the functional features of Penicillium oxalicum transcriptional activator XlnR. The yeast reporter system was used to identify transcriptional activation domain of XlnR in P. oxalicum. The expression cassette was introduced into the xlnR locus of P. oxalicum by homologous recombination. In this study, several putative structural domains in P. oxalicum XlnR were predicted by bioinformatics analysis, and the transcriptional activation domain (351-694 region) was identified in XlnR relying on reporter gene system in yeast. In addition, the amino acid at XlnR 871 site (alanine) located in the regulatory region could influence the regulatory activity of XlnR directly. When the alanine at XlnR 871 site was replaced by stronger hydrophobic amino acid (e.g. valine or isoleucine), the regulatory activity will be greatly improved, especially for the regulation of hemicellulase genes expression. When alanine at XlnR 871 site was mutated to a hydrophilic amino acid (e.g. aspartic acid or arginine), the regulatory activity of XlnR will be reduced. The 351-694 region of P. oxalicum XlnR was identified as transcriptional activation domain, and the regulatory activity of XlnR was greatly influenced by hydrophobicity of amino acid at 871 site of XlnR in P. oxalicum. The results will provide an effective target site to regulate the activity of XlnR and improve cellulase production of P. oxalicum.
Substances chimiques
Transcription Factors
0
Cellulase
EC 3.2.1.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1112-1120Subventions
Organisme : The National Key Research and Development Program of China
ID : 2021YFC2101300
Organisme : National Natural Science Foundation of China
ID : 32002143
Organisme : National Natural Science Foundation of China
ID : 31971387
Organisme : Youth Foundation of the Shanxi Science and Technology Department
ID : 201901D211375
Organisme : Major basic research projects of Natural Science Foundation of Shandong Province
ID : ZR2019ZD19
Organisme : Animal Husbandry and '1331 project' Key Discipline Construction program of Shanxi Province
ID : 2020BQ07
Organisme : Science and Technology Innovation Fund Project of Shanxi Agricultural University
ID : 2020BQ07
Informations de copyright
© 2021 Society for Applied Microbiology.
Références
Amore, A., Giacobbe, S. & Faraco, V. (2013) Regulation of cellulase and hemicellulase gene expression in fungi. Current Genomics, 14, 230-249.
Bischof, R.H., Ramoni, J. & Seiboth, B. (2016) Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei. Microbial Cell Factories, 15, 106.
Coradetti, S.T., Craig, J.P., Xiong, Y., Shock, T., Tian, C. & Glass, N.L. (2012) Conserved and essential transcription factors for cellulase gene expression in ascomycete fungi. Proceedings of the National Academy of Sciences, 109, 7397-7402.
Craig, J.P., Coradetti, S.T., Starr, T.L. & Glass, N.L. (2015) Direct target network of the Neurospora crassa plant cell wall deconstruction regulators CLR-1, CLR-2, and XLR-1. MBio, 6(5), e01452-15. https://doi.org/10.1128/mBio.01452-15
Derntl, C., Gudynaite-Savitch, L., Calixte, S., White, T., Mach, R.L. & Mach-Aigner, A.R. (2013) Mutation of the xylanase regulator 1 causes a glucose blind hydrolase expressing phenotype in industrially used Trichoderma strains. Biotechnology for Biofuels, 6, 62.
Dowzer, C.E. & Kelly, J.M. (1991) Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Molecular and Cellular Biology, 11, 5701-5709.
Ebbole, D.J. (1998) Carbon catabolite repression of gene expression and conidiation in Neurospora crassa. Fungal Genetics and Biology, 25, 15-21.
Gao, L., Li, Z., Xia, C., Qu, Y., Liu, M., Yang, P. et al. (2017a) Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum. Biotechnology for Biofuels, 10, 100.
Gao, L., Xia, C., Xu, J., Li, Z., Yu, L., Liu, G. et al. (2017b) Constitutive expression of chimeric transcription factors enables cellulase synthesis under non-inducing conditions in Penicillium oxalicum. Biotechnology Journal, 12, https://doi.org/10.1002/biot.201700119
Gao, L., Xu, Y., Song, X., Li, S., Xia, C., Xu, J., et al. (2019) Deletion of the middle region of the transcription factor ClrB in Penicillium oxalicum enables cellulase production in the presence of glucose. Journal of Biological Chemistry, 294, 18685-18697.
Glass, N.L., Schmoll, M., Cate, J.H. & Coradetti, S. (2013) Plant cell wall deconstruction by ascomycete fungi. Annual Review of Microbiology, 67, 477-498.
Gusakov, A.V. (2011) Alternatives to Trichoderma reesei in biofuel production. Trends in Biotechnology, 29, 419-425.
Hasper, A.A., Trindade, L.M., van der Veen, D., van Ooyen, A.J.J. & de Graaff, L.H. (2004) Functional analysis of the transcriptional activator XlnR from Aspergillus niger. Microbiology, 150, 1367-1375.
Ilmen, M., Thrane, C. & Penttila, M. (1996) The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Molecular and General Genetics, 251, 451-460.
Klaubauf, S., Narang, H.M., Post, H., Zhou, M., Brunner, K., Mach-Aigner, A.R. et al. (2014) Similar is not the same: differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi. Fungal Genetics and Biology, 72, 73-81.
Li, Z., Yao, G., Wu, R., Gao, L., Kan, Q., Liu, M. et al. (2015) Synergistic and dose-controlled regulation of cellulase gene expression in Penicillium oxalicum. PLoS Genetics, 11, e1005509.
Mach-Aigner, A.R., Pucher, M.E., Steiger, M.G., Bauer, G.E., Preis, S.J. & Mach, R.L. (2008) Transcriptional regulation of xyr1, encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina. Applied and Environment Microbiology, 74, 6554-6562.
MacPherson, S., Larochelle, M. & Turcotte, B. (2006) A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiology and Molecular Biology Reviews, 70, 583-604.
Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426-428. https://doi.org/10.1021/ac60147a030
Ogawa, M., Kobayashi, T. & Koyama, Y. (2012) ManR, a novel Zn(II)2Cys6 transcriptional activator, controls the beta-mannan utilization system in Aspergillus oryzae. Fungal Genetics and Biology, 49, 987-995.
Qin, Y.Q., Zheng, K., Liu, G.D., Chen, M. & Qu, Y.B. (2013) Improved cellulolytic efficacy in Penicillium decumbens via heterologous expression of Hypocrea jecorina endoglucanase II. Archives of Biological Sciences, 65, 305-314.
Raulo, R., Kokolski, M. & Archer, D.B. (2016) The roles of the zinc finger transcription factors XlnR, ClrA and ClrB in the breakdown of lignocellulose by Aspergillus niger. AMB Express, 6, 5.
Rauscher, R., Wurleitner, E., Wacenovsky, C., Aro, N., Stricker, A.R., Zeilinger, S. et al. (2006) Transcriptional regulation of xyn1, encoding xylanase I, in Hypocrea jecorina. Eukaryotic Cell, 5, 447-456.
Schjerling, P. & Holmberg, S. (1996) Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators. Nucleic Acids Research, 24, 4599-4607.
Stricker, A.R., Grosstessner-Hain, K., Wurleitner, E. & Mach, R.L. (2006) Xyr1 (xylanase regulator 1) regulates both the hydrolytic enzyme system and D-xylose metabolism in Hypocrea jecorina. Eukaryotic Cell, 5, 2128-2137.
Sun, J., Tian, C., Diamond, S. & Glass, N.L. (2012) Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa. Eukaryotic Cell, 11, 482-493.
Tjian, R. & Maniatis, T. (1994) Transcriptional activation: a complex puzzle with few easy pieces. Cell, 77, 5-8. https://doi.org/10.1016/0092-8674(94)90227-5
Turcotte, B., Akache, B. & MacPherson, S. (2004) The zinc cluster proteins: a yeast family of transcriptional regulators. In: Pandalai, S.G. (Ed.) Recent developments in nucleic acid research, vol 1. Trivandrum, Kerala, India: Transworld Research Network, pp. 233-249.
van Peij, N.N., Visser, J. & de Graaff, L.H. (1998) Isolation and analysis of xlnR, encoding a transcriptional activator co-ordinating xylanolytic expression in Aspergillus niger. Molecular Microbiology, 27, 131-142.
Vogel, H.J. (1956) A convenient growth medium for Neurospora crassa. Microbial Genetics Bulletin, 13, 42-43.
Voss, T.C. & Hager, G.L. (2014) Dynamic regulation of transcriptional states by chromatin and transcription factors. Nature Reviews Genetics, 15, 69-81.
Wurleitner, E., Pera, L., Wacenovsky, C., Cziferszky, A., Zeilinger, S., Kubicek, C.P. et al. (2003) Transcriptional regulation of xyn2 in Hypocrea jecorina. Eukaryotic Cell, 2, 150-158.
Xia, C., Li, Z., Xu, Y., Yang, P., Gao, L., Yan, Q. et al. (2019) Introduction of heterologous transcription factors and their target genes into Penicillium oxalicum leads to increased lignocellulolytic enzyme production. Applied Microbiology and Biotechnology, 103, 2675-2687.
Yu, J.H., Hamari, Z., Han, K.H., Seo, J.A., Reyes-Dominguez, Y. & Scazzocchio, C. (2004) Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genetics and Biology, 41, 973-981.
Zhang, X.Y., Li, Y.H., Zhao, X.Q. & Bai, F.W. (2017) Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator. Bioresource Technology, 223, 317-322.