A mouse model of ankle-subtalar joint complex instability induced post-traumatic osteoarthritis.
Ankle instability
Biomechanics
Cervical ligament
Gait
Subtalar instability
Journal
Journal of orthopaedic surgery and research
ISSN: 1749-799X
Titre abrégé: J Orthop Surg Res
Pays: England
ID NLM: 101265112
Informations de publication
Date de publication:
01 Sep 2021
01 Sep 2021
Historique:
received:
01
06
2021
accepted:
20
08
2021
entrez:
2
9
2021
pubmed:
3
9
2021
medline:
15
1
2022
Statut:
epublish
Résumé
Ankle-subtalar joint complex instability is not uncommonly presented in the clinic, but symptoms and signs similar to other conditions can easily lead to its misdiagnosis. Due to the lack of appropriate animal models, research on ankle-subtalar joint complex instability is limited. The aims of the present study were to establish an animal model of ankle-subtalar joint complex instability in mice and to explore its relationship with post-traumatic osteoarthritis (PTOA). Twenty-one male C57BL/6J mice were randomly divided into three groups: SHAM group (sham surgery group), transected cervical ligament + anterior talofibular ligament (CL+ATFL) group, and transected cervical ligament + deltoid ligament (CL+DL) group. Two weeks after surgery, all mice underwent cage running training. Balance beam and gait tests were used to evaluate the changes in self-movement in the mice after ankle-subtalar ligament injury. Micro-CT and histological staining were used to evaluate the progress of PTOA. Compared with the SHAM group, balance and gait were affected in the ligament transection group. Twelve weeks after surgery, the time required to cross the balance beam in the CL+ATFL group was 35.1% longer and the mice slipped 3.6-fold more often than before surgery, and the mean step length on the right side was 7.2% smaller than that in the SHAM group. The time required to cross the balance beam in the CL+DL group was 32.1% longer and the mice slipped 3-fold more often than prior to surgery, and the average step length on the right side was 5.6% smaller than that in the SHAM group. CT images indicated that 28.6% of the mice in the CL+DL group displayed dislocation of the talus. Tissue staining suggested that articular cartilage degeneration occurred in mice with ligament transection 12 weeks after surgery. Transected mice in the CL+ATFL and CL+DL groups displayed mechanical instability of the ankle-subtalar joint complex, and some mice in the CL+DL group also suffered from talus dislocation due to ligament injury leading to loss of stability of the bone structure. In addition, as time progressed, the articular cartilage displayed degenerative changes, which affected the ability of animals to move normally.
Sections du résumé
BACKGROUND
BACKGROUND
Ankle-subtalar joint complex instability is not uncommonly presented in the clinic, but symptoms and signs similar to other conditions can easily lead to its misdiagnosis. Due to the lack of appropriate animal models, research on ankle-subtalar joint complex instability is limited. The aims of the present study were to establish an animal model of ankle-subtalar joint complex instability in mice and to explore its relationship with post-traumatic osteoarthritis (PTOA).
METHODS
METHODS
Twenty-one male C57BL/6J mice were randomly divided into three groups: SHAM group (sham surgery group), transected cervical ligament + anterior talofibular ligament (CL+ATFL) group, and transected cervical ligament + deltoid ligament (CL+DL) group. Two weeks after surgery, all mice underwent cage running training. Balance beam and gait tests were used to evaluate the changes in self-movement in the mice after ankle-subtalar ligament injury. Micro-CT and histological staining were used to evaluate the progress of PTOA.
RESULTS
RESULTS
Compared with the SHAM group, balance and gait were affected in the ligament transection group. Twelve weeks after surgery, the time required to cross the balance beam in the CL+ATFL group was 35.1% longer and the mice slipped 3.6-fold more often than before surgery, and the mean step length on the right side was 7.2% smaller than that in the SHAM group. The time required to cross the balance beam in the CL+DL group was 32.1% longer and the mice slipped 3-fold more often than prior to surgery, and the average step length on the right side was 5.6% smaller than that in the SHAM group. CT images indicated that 28.6% of the mice in the CL+DL group displayed dislocation of the talus. Tissue staining suggested that articular cartilage degeneration occurred in mice with ligament transection 12 weeks after surgery.
CONCLUSIONS
CONCLUSIONS
Transected mice in the CL+ATFL and CL+DL groups displayed mechanical instability of the ankle-subtalar joint complex, and some mice in the CL+DL group also suffered from talus dislocation due to ligament injury leading to loss of stability of the bone structure. In addition, as time progressed, the articular cartilage displayed degenerative changes, which affected the ability of animals to move normally.
Identifiants
pubmed: 34470616
doi: 10.1186/s13018-021-02683-0
pii: 10.1186/s13018-021-02683-0
pmc: PMC8408979
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
541Subventions
Organisme : National Natural Science Foundation of China
ID : 11572211
Organisme : Key Technology Research and Development Program of Shandong
ID : 2018YFB1107000
Informations de copyright
© 2021. The Author(s).
Références
Injury. 2017 Jul;48(7):1678-1683
pubmed: 28438418
Life Sci. 2010 Mar 27;86(13-14):538-43
pubmed: 20188111
J Sci Med Sport. 2015 Mar;18(2):128-32
pubmed: 24631124
Int Orthop. 2020 Feb;44(2):341-347
pubmed: 31776609
Clin Orthop Relat Res. 2007 Feb;455:169-72
pubmed: 17279044
J Athl Train. 2019 Jun;54(6):603-610
pubmed: 31135209
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S17-23
pubmed: 20864019
Foot Ankle Clin. 2018 Sep;23(3):397-413
pubmed: 30097081
Phys Sportsmed. 2018 Feb;46(1):116-122
pubmed: 29171312
J Orthop Res. 2011 Oct;29(10):1459-64
pubmed: 21445995
Med Clin North Am. 2014 Mar;98(2):313-29
pubmed: 24559877
Curr Med Res Opin. 2018 Jul;34(7):1217-1229
pubmed: 28952378
Osteoarthritis Cartilage. 2006 Jan;14(1):13-29
pubmed: 16242352
Med Sci Sports Exerc. 2015 Apr;47(4):866-72
pubmed: 25083728
J Sport Health Sci. 2019 Nov;8(6):548-554
pubmed: 31720066
Osteoarthritis Cartilage. 2016 Apr;24(4):688-97
pubmed: 26596790
Med Sci Sports Exerc. 2013 Aug;45(8):1623-8
pubmed: 23439419
Neurosci Lett. 2008 Sep 12;442(2):161-4
pubmed: 18620022
Knee Surg Sports Traumatol Arthrosc. 2018 Jul;26(7):2095-2102
pubmed: 28439639
Foot Ankle Int. 2017 Jul;38(7):785-790
pubmed: 28447855
Eur J Trauma Emerg Surg. 2015 Dec;41(6):623-9
pubmed: 26510942
Knee Surg Sports Traumatol Arthrosc. 2016 Apr;24(4):1029-39
pubmed: 26869035
J Bone Joint Surg Am. 2015 Mar 18;97(6):513-20
pubmed: 25788309
Foot Ankle Int. 2012 Feb;33(2):151-60
pubmed: 22381348
J Athl Train. 2018 Mar;53(3):249-254
pubmed: 29412694
J Bone Miner Res. 2010 Jul;25(7):1468-86
pubmed: 20533309
J Athl Train. 2019 Jul;54(7):801-807
pubmed: 31343261
Foot Ankle Int. 2000 Jun;21(6):486-91
pubmed: 10884108
Biomed Pharmacother. 2020 Jul;127:110144
pubmed: 32330796
Foot Ankle Clin. 2002 Sep;7(3):577-609
pubmed: 12512411
J Athl Train. 2019 Jun;54(6):589-602
pubmed: 31184957