Methods to Synthesize and Assemble Recombinant Keratins.
Characterization
Intermediate filament
Keratin
Recombinant keratin protein
Self-assembly
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
2
9
2021
pubmed:
3
9
2021
medline:
15
1
2022
Statut:
ppublish
Résumé
Keratin, as one of the most abundant and underexploited protein sources, is a ubiquitous biological material that commonly exists in epithelial cells. Due to the excellent biocompatibility and biodegradability, keratin is widely used in biomedical applications. Previously, these biomaterials were prepared by dissolving and extracting the keratinous materials. However, the keratins obtained by direct extraction is not pure and contain many by-products. Moreover, natural keratins suffer from limited sequence tenability. In comparison, the recombinant keratin proteins produced by recombinant technology can overcome these drawbacks while maintaining the desired chemical and physical characteristics of natural keratins. Accordingly, this chapter mainly introduces the experimental protocols of the recombination of keratin. As these recombinant keratins are often used for assembly of intermediate filaments (IFs) in vitro, assembly protocols are also introduced in this chapter.
Identifiants
pubmed: 34472059
doi: 10.1007/978-1-0716-1574-4_10
doi:
Substances chimiques
Recombinant Proteins
0
Keratins
68238-35-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105-112Informations de copyright
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.
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