Cold Regulation of Genes Encoding Ion Transport Systems in the Oligotrophic Bacterium Caulobacter crescentus.

Bacterial Proteins / genetics Caulobacter crescentus / chemistry Cold Temperature Gene Expression Regulation, Bacterial Ion Transport Iron / metabolism RNA, Bacterial / genetics RNA, Untranslated / genetics Regulon Repressor Proteins / genetics
Caulobacter crescentus cation homeostasis cold adaptation potassium transport transcriptomic analysis

Journal

Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614

Informations de publication

Date de publication:
03 09 2021
Historique:
entrez: 4 9 2021
pubmed: 5 9 2021
medline: 18 1 2022
Statut: ppublish

Résumé

In this study, we characterize the response of the free-living oligotrophic alphaproteobacterium Caulobacter crescentus to low temperatures by global transcriptomic analysis. Our results showed that 656 genes were upregulated and 619 were downregulated at least 2-fold after a temperature downshift. The identified differentially expressed genes (DEG) belong to several functional categories, notably inorganic ion transport and metabolism, and a subset of these genes had their expression confirmed by reverse transcription quantitative real-time PCR (RT-qPCR). Several genes belonging to the ferric uptake regulator (Fur) regulon were downregulated, indicating that iron homeostasis is relevant for adaptation to cold. Several upregulated genes encode proteins that interact with nucleic acids, particularly RNA:

Identifiants

DOI: 10.1128/Spectrum.00710-21 PMID: 34479415 PMC: PMC8552747
pubmed: 34479415
doi: 10.1128/Spectrum.00710-21
pmc: PMC8552747
doi:

Substances chimiques

Bacterial Proteins 0
RNA, Bacterial 0
RNA, Untranslated 0
Repressor Proteins 0
ferric uptake regulating proteins, bacterial 0
Iron E1UOL152H7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0071021

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Auteurs

Hugo L de Araújo (HL)

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
ORCID: 0000-0002-4236-625X

Bianca P Martins (BP)

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.

Alexandre M Vicente (AM)

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
ORCID: 0000-0001-9142-675X

Alan P R Lorenzetti (APR)

Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
ORCID: 0000-0002-0291-248X

Tie Koide (T)

Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
ORCID: 0000-0003-4760-2423

Marilis V Marques (MV)

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
ORCID: 0000-0003-1542-8221

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Caulobacteraceae › Caulobacter › Caulobacter crescentus › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Basse température › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Métabolisme › Transport biologique › Transport des ions › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Fer › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN bactérien › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Régulon › Cold Regulation of Genes Encoding Ion...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines de répression › Cold Regulation of Genes Encoding Ion...