Brown adipose tissue monocytes support tissue expansion.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 09 2021
06 09 2021
Historique:
received:
28
01
2021
accepted:
12
08
2021
entrez:
7
9
2021
pubmed:
8
9
2021
medline:
21
9
2021
Statut:
epublish
Résumé
Monocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments demonstrated that the BAT macrophage pool requires constant replenishment from monocytes. Using a genetic model of BAT expansion, we found that brown fat monocyte numbers were selectively increased in this scenario. This observation was confirmed using a CCR2-binding radiotracer and positron emission tomography. Importantly, in line with their tissue recruitment, blood monocyte counts were decreased while bone marrow hematopoiesis was not affected. Monocyte depletion prevented brown adipose tissue expansion and altered its architecture. Podoplanin engagement is strictly required for BAT expansion. Together, these data redefine the diversity of immune cells in the BAT and emphasize the role of monocyte recruitment for tissue remodeling.
Identifiants
pubmed: 34489438
doi: 10.1038/s41467-021-25616-1
pii: 10.1038/s41467-021-25616-1
pmc: PMC8421389
doi:
Substances chimiques
Adiponectin
0
Adipoq protein, mouse
0
Ccr2 protein, mouse
0
Gp38 protein, mouse
0
Membrane Glycoproteins
0
Receptors, CCR2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5255Subventions
Organisme : NHLBI NIH HHS
ID : K99 HL138163
Pays : United States
Organisme : NHLBI NIH HHS
ID : R00 HL138163
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021. The Author(s).
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