Selective Binding of Heparin/Heparan Sulfate Oligosaccharides to Factor H and Factor H-Related Proteins: Therapeutic Potential for C3 Glomerulopathies.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2021
Historique:
received: 05 03 2021
accepted: 02 08 2021
entrez: 7 9 2021
pubmed: 8 9 2021
medline: 27 10 2021
Statut: epublish

Résumé

Complement dysregulation is characteristic of the renal diseases atypical hemolytic uremic syndrome (aHUS) and complement component 3 glomerulopathy (C3G). Complement regulatory protein Factor H (FH) inhibits complement activity, whereas FH-related proteins (FHRs) lack a complement regulatory domain. FH and FHRs compete for binding to host cell glycans, in particular heparan sulfates (HS). HS is a glycosaminoglycan with an immense structural variability, where distinct sulfation patterns mediate specific binding of proteins. Mutations in FH, FHRs, or an altered glomerular HS structure may disturb the FH : FHRs balance on glomerular endothelial cells, thereby leading to complement activation and the subsequent development of aHUS/C3G. In this study, we aimed to identify specific HS structures that could specifically compete off FHRs from HS glycocalyx (HS

Identifiants

pubmed: 34489931
doi: 10.3389/fimmu.2021.676662
pmc: PMC8416517
doi:

Substances chimiques

CFHR5 protein, human 0
Complement C3b 80295-43-8
Complement Factor H 80295-65-4
Heparin 9005-49-6
Complement System Proteins 9007-36-7
Heparitin Sulfate 9050-30-0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

676662

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK110023
Pays : United States

Informations de copyright

Copyright © 2021 Loeven, Maciej-Hulme, Yanginlar, Hubers, Kellenbach, de Graaf, van Kuppevelt, Wetzels, Rabelink, Smith and van der Vlag.

Déclaration de conflit d'intérêts

Author EK was employed by company Aspen API. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Markus A Loeven (MA)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Marissa L Maciej-Hulme (ML)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Cansu Yanginlar (C)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Melanie C Hubers (MC)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Edwin Kellenbach (E)

Biochemical Technical Support Aspen Oss, Oss, Netherlands.

Mark de Graaf (M)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Toin H van Kuppevelt (TH)

Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Jack Wetzels (J)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

Ton J Rabelink (TJ)

Department of Nephrology and Einthoven Laboratory for Vascular Medicine, Leiden University Medical Center, Leiden, Netherlands.

Richard J H Smith (RJH)

Departments of Internal Medicine and Otolaryngology, Carver College of Medicine, Iowa City, IA, United States.

Johan van der Vlag (J)

Department of Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.

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