Deferoxamine accelerates endothelial progenitor cell senescence and compromises angiogenesis.
Aging
/ metabolism
Animals
Cell Proliferation
Cells, Cultured
Cellular Senescence
Deferoxamine
/ pharmacology
Endothelial Progenitor Cells
/ cytology
Hindlimb
/ blood supply
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Mitochondria
/ metabolism
Neovascularization, Pathologic
Rats
Rats, Sprague-Dawley
Telomerase
/ metabolism
angiogenesis
deferoxamine
endothelial progenitor cell
senescence
Journal
Aging
ISSN: 1945-4589
Titre abrégé: Aging (Albany NY)
Pays: United States
ID NLM: 101508617
Informations de publication
Date de publication:
11 09 2021
11 09 2021
Historique:
received:
19
02
2021
accepted:
02
08
2021
pubmed:
12
9
2021
medline:
15
1
2022
entrez:
11
9
2021
Statut:
ppublish
Résumé
Senescence reduces the circulating number and angiogenic activity of endothelial progenitor cells (EPCs), and is associated with aging-related vascular diseases. However, it is very time-consuming to obtain aged cells (~1 month of repeated replication) or animals (~2 years) for senescence studies. Here, we established an accelerated senescence model by treating EPCs with deferoxamine (DFO), an FDA-approved iron chelator. Four days of low-dose (3 μM) DFO induced senescent phenotypes in EPCs, including a senescent pattern of protein expression, impaired mitochondrial bioenergetics, altered mitochondrial protein levels and compromised angiogenic activity. DFO-treated early EPCs from young and old donors (< 35 vs. > 70 years old) displayed similar senescent phenotypes, including elevated senescence-associated β-galactosidase activity and reduced relative telomere lengths, colony-forming units and adenosine triphosphate levels. To validate this accelerated senescence model
Identifiants
pubmed: 34508614
pii: 203469
doi: 10.18632/aging.203469
pmc: PMC8457614
doi:
Substances chimiques
Telomerase
EC 2.7.7.49
Deferoxamine
J06Y7MXW4D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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