XIST Inhibition Attenuates Calcium Oxalate Nephrocalcinosis-Induced Renal Inflammation and Oxidative Injury via the miR-223/NLRP3 Pathway.
Animals
Calcium Oxalate
/ administration & dosage
Humans
Inflammation
/ drug therapy
Male
Mice
Mice, Inbred C57BL
MicroRNAs
/ metabolism
NLR Family, Pyrin Domain-Containing 3 Protein
/ metabolism
Nephrocalcinosis
/ drug therapy
Oxidative Stress
/ drug effects
RNA, Long Noncoding
/ antagonists & inhibitors
Transfection
Journal
Oxidative medicine and cellular longevity
ISSN: 1942-0994
Titre abrégé: Oxid Med Cell Longev
Pays: United States
ID NLM: 101479826
Informations de publication
Date de publication:
2021
2021
Historique:
received:
21
04
2021
revised:
04
07
2021
accepted:
06
08
2021
entrez:
13
9
2021
pubmed:
14
9
2021
medline:
19
2
2022
Statut:
epublish
Résumé
The roles of the lncRNA X inactive specific transcript (XIST) in many diseases, including cancers and inflammatory sickness, have been previously elucidated. However, renal calculus remained poorly understood. In this study, we revealed the potential effects of XIST on kidney stones that were exerted via inflammatory response and oxidative stress mechanisms. We established a glyoxylate-induced calcium oxalate (CaOx) stone mouse model and exposed HK-2 cells to calcium oxalate monohydrate (COM). The interactions among XIST, miR-223-3p, and NOD-like receptor protein 3 (NLRP3) and their respective effects were determined by RNAs and protein expression, luciferase activity, and immunohistochemistry (IHC) assays. Cell necrosis, reactive oxygen species (ROS) generation, and inflammatory responses were detected after silencing XIST, activating and inhibiting miR-223-3p, and both knocking down XIST and activating miR-223-3p in vitro and in vivo. The XIST, NLRP3, caspase-1, and IL-1
Identifiants
pubmed: 34512861
doi: 10.1155/2021/1676152
pmc: PMC8429007
doi:
Substances chimiques
MIRN223 microRNA, human
0
MIRN223 microRNA, mouse
0
MicroRNAs
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
NLRP3 protein, human
0
Nlrp3 protein, mouse
0
RNA, Long Noncoding
0
XIST non-coding RNA
0
Calcium Oxalate
2612HC57YE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1676152Informations de copyright
Copyright © 2021 Peng Lv et al.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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