A regulatory cascade controls Staphylococcus aureus pathogenicity island activation.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
05
04
2020
accepted:
29
07
2021
pubmed:
15
9
2021
medline:
5
11
2021
entrez:
14
9
2021
Statut:
ppublish
Résumé
Staphylococcal pathogenicity islands (SaPIs) are a family of closely related mobile chromosomal islands that encode and disseminate the superantigen toxins, toxic shock syndrome toxin 1 and superantigen enterotoxin B (SEB). They are regulated by master repressors, which are counteracted by helper phage-encoded proteins, thereby inducing their excision, replication, packaging and intercell transfer. SaPIs are major components of the staphylococcal mobilome, occupying five chromosomal att sites, with many strains harbouring two or more. As regulatory interactions between co-resident SaPIs could have profound effects on the spread of superantigen pathobiology, we initiated the current study to search for such interactions. Using classical genetics, we found that, with one exception, their regulatory systems do not cross-react. The exception was SaPI3, which was originally considered defective because it could not be mobilized by any known helper phage. We show here that SaPI3 has an atypical regulatory module and is induced not by a phage but by many other SaPIs, including SaPI2, SaPIbov1 and SaPIn1, each encoding a conserved protein, Sis, which counteracts the SaPI3 repressor, generating an intracellular regulatory cascade: the co-resident SaPI, when conventionally induced by a helper phage, expresses its sis gene which, in turn, induces SaPI3, enabling it to spread. Using bioinformatics analysis, we have identified more than 30 closely related coancestral SEB-encoding SaPI3 relatives occupying the same att site and controlled by a conserved regulatory module, immA-immR-str'. This module is functionally analogous but unrelated to the typical SaPI regulatory module, stl-str. As SaPIs are phage satellites, SaPI3 and its relatives are SaPI satellites.
Identifiants
pubmed: 34518655
doi: 10.1038/s41564-021-00956-2
pii: 10.1038/s41564-021-00956-2
pmc: PMC7611864
mid: EMS136588
doi:
Substances chimiques
Bacterial Proteins
0
DNA, Bacterial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1300-1308Subventions
Organisme : Medical Research Council
ID : MR/S00940X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V000772/1
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI139613
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S003835/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 201531/Z/16/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N002873/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 201531
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M003876/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S00940X/2
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature Limited.
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