A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis.
Bacillus subtilis
CRISPR-Cas9
allelic replacement
genetics
genome editing
transformation
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
31 10 2021
31 10 2021
Historique:
pubmed:
16
9
2021
medline:
1
2
2022
entrez:
15
9
2021
Statut:
ppublish
Résumé
The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction. Conventional CRISPR-Cas9 editing strategies require design and molecular cloning of an appropriate guide RNA (gRNA) to target genome cleavage and a repair template for introduction of the desired site-specific genome modification. Here, we present a streamlined method that leverages the existing collection of nearly 4,000 Bacillus subtilis strains (the BKE collection) with individual genes replaced by an integrated erythromycin (
Identifiants
pubmed: 34523974
doi: 10.1128/Spectrum.00754-21
pmc: PMC8557940
doi:
Substances chimiques
Bacterial Proteins
0
YqgE protein, Bacillus subtilis
0
RNA, Guide, CRISPR-Cas Systems
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0075421Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM122461
Pays : United States
Références
J Microbiol Biotechnol. 2010 Jan;20(1):45-53
pubmed: 20134232
Appl Environ Microbiol. 2020 Aug 18;86(17):
pubmed: 32631858
Elife. 2020 Sep 08;9:
pubmed: 32897856
J Biotechnol. 2018 Oct 20;284:57-62
pubmed: 30092237
Appl Environ Microbiol. 2008 Sep;74(17):5556-62
pubmed: 18641148
PLoS One. 2014 May 29;9(5):e98360
pubmed: 24874808
PLoS Genet. 2018 Sep 24;14(9):e1007689
pubmed: 30248093
Genes Genet Syst. 2009 Aug;84(4):315-8
pubmed: 20057169
Biotechnol Bioeng. 2020 Jun;117(6):1805-1816
pubmed: 32077487
PLoS Genet. 2019 Oct 18;15(10):e1008263
pubmed: 31626625
Microb Cell Fact. 2013 Jan 14;12:3
pubmed: 23311580
Annu Rev Food Sci Technol. 2020 Mar 25;11:295-318
pubmed: 31905010
Cell. 2016 Jun 2;165(6):1493-1506
pubmed: 27238023
PLoS Genet. 2019 Oct 7;15(10):e1008434
pubmed: 31589605
Cell Syst. 2020 Nov 18;11(5):523-535.e9
pubmed: 33080209
Microbiology (Reading). 1998 Nov;144 ( Pt 11):3097-3104
pubmed: 9846745
Appl Environ Microbiol. 2020 Dec 17;87(1):
pubmed: 33097498
Nat Rev Genet. 2015 May;16(5):299-311
pubmed: 25854182
Appl Environ Microbiol. 2016 Aug 15;82(17):5421-7
pubmed: 27342565
Bio Protoc. 2017 Apr 20;7(8):
pubmed: 28706963
Science. 2019 Jul 5;365(6448):48-53
pubmed: 31171706
J Biochem. 2006 Feb;139(2):203-11
pubmed: 16452308
J Microbiol Biotechnol. 2021 Jul 28;31(7):903-911
pubmed: 34261850
World J Microbiol Biotechnol. 2018 Sep 29;34(10):153
pubmed: 30269229
Yeast. 1996 Mar 15;12(3):259-65
pubmed: 8904338
J Bacteriol. 2020 Jan 2;202(2):
pubmed: 31685536
Biochem Mol Biol Educ. 2018 Mar;46(2):195-205
pubmed: 29381252
Biotechnol Lett. 2019 Feb;41(2):293-303
pubmed: 30547274
Mol Microbiol. 2019 Dec;112(6):1635-1644
pubmed: 31502713
Appl Environ Microbiol. 2016 Jul 29;82(16):4876-95
pubmed: 27260361
Cell Syst. 2017 Mar 22;4(3):291-305.e7
pubmed: 28189581
Science. 2014 Nov 28;346(6213):1258096
pubmed: 25430774
Front Microbiol. 2017 Jun 23;8:1167
pubmed: 28690606
Nature. 2019 Jul;571(7764):219-225
pubmed: 31189177
Nat Commun. 2019 Apr 4;10(1):1526
pubmed: 30948730