A time-resolved fluorescence resonance energy transfer screening assay for discovery of protein-protein interaction modulators.
Cancer
High Throughput Screening
Molecular/Chemical Probes
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
17
9
2021
Statut:
epublish
Résumé
Protein-protein interactions (PPIs) have emerged as promising yet challenging therapeutic targets. A robust bioassay is required for rapid PPI modulator discovery. Here, we present a time-resolved Förster's (fluorescence) resonance energy transfer assay protocol for PPI modulator screening in a 1536-well plate format. We use hypomorph SMAD4
Identifiants
pubmed: 34527960
doi: 10.1016/j.xpro.2021.100804
pii: S2666-1667(21)00510-4
pmc: PMC8433285
doi:
Substances chimiques
SMAD4 protein, human
0
Smad4 Protein
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100804Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008602
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008169
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002378
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA217875
Pays : United States
Organisme : NCI NIH HHS
ID : R37 CA255459
Pays : United States
Informations de copyright
© 2021.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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