A strain of highly pathogenic porcine reproductive and respiratory syndrome virus: genomic characterization, pathogenicity, and construction of an infectious full-length cDNA clone.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
02
12
2020
accepted:
08
07
2021
pubmed:
17
9
2021
medline:
16
10
2021
entrez:
16
9
2021
Statut:
ppublish
Résumé
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV), which inflicts major economic losses on the global pig farming industry. Based on its similarity to highly pathogenic strains, the GDzj strain isolated in this study was predicted to be highly pathogenic. We therefore analyzed the pathogenicity of this strain experimentally in piglets. All piglets challenged with this virus experienced fever or high fever, loss of appetite, decreased food intake, daily weight loss, shortness of breath, and listlessness, and the necropsy results showed that they had experienced severe interstitial pneumonia. We then used the BAC system to construct a full-length cDNA infectious clone of GDzj, and the rescued virus displayed in vitro proliferation characteristics similar to those of the parental PRRSV strain. In summary, we successfully isolated a highly pathogenic PRRSV strain and constructed a full-length infectious cDNA clone from it, thereby providing an effective reverse genetics platform for further study of viral pathogenesis.
Identifiants
pubmed: 34529151
doi: 10.1007/s00705-021-05212-w
pii: 10.1007/s00705-021-05212-w
doi:
Substances chimiques
DNA, Complementary
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3127-3141Subventions
Organisme : the National Key Research and Development Program of China
ID : 2018YFD0501100
Organisme : Major animal disease prevention and control technology research and promotion projects
ID : 201817SY0002
Organisme : Guangdong Province Key Field R&D Program Project
ID : 2019B020211003
Organisme : the 2020 Youth Backbone Training Plan of Henan University of Higher Education
ID : ZDXK201702
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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