Lower p66Shc promoter methylation in subjects with chronic renal failure.
Adult
Aged
Aged, 80 and over
Case-Control Studies
DNA Methylation
/ genetics
Female
Humans
Inflammation
/ genetics
Kidney Function Tests
Male
Malondialdehyde
/ metabolism
Middle Aged
Oxidative Stress
/ genetics
Promoter Regions, Genetic
Renal Insufficiency, Chronic
/ genetics
Src Homology 2 Domain-Containing, Transforming Protein 1
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
17
06
2021
accepted:
24
08
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
18
11
2021
Statut:
epublish
Résumé
To determine the correlation between DNA methylation of p66Shc promoter and some markers of inflammatory and oxidative stress in chronic renal failure (CRF) patients compared with healthy subjects. An observational cross-sectional study was conducted in the nephrology department at Sidi Bouzid Regional Hospital (Tunisia). In total, 39 patients with CRF and 37 healthy subjects were included. Several biochemical parameters were measured. Furthermore, markers of the oxidative and inflammatory status (MDA, TAS, SOD, and CRP) were evaluated. The p66Shc methylation status was determined using the methylation-specific PCR. Our results showed that levels of blood glucose, urea, creatinine, uric acid, ChT, TG, albuminuria, CRP and MDA were significantly elevated in CRF patients compared to controls. Furthermore, p66Shc promoter region was highly demethylated in CRF patients compared to healthy controls (84% vs 4%). Our data showed a positive correlation between p66Shc hypomethylation and levels of MDA (r = 0.93; p<0, 05) and CRP (r = 0.89; P <0, 05), as well as a significant negative correlation between p66Shc hypomethylation, TAS (r = -0.76; P <0, 05) and SOD (r = -0.77; p<0, 05) levels. Similarly, there was a positive correlation between p66Shc hypomethylation and the disease stages. Importantly, multiple regression analysis showed that p66shc DNA hypomethylation remains strongly correlated with MDA, CRP and stages of CRF. This study indicates that the DNA hypomethylation of p66shc promoter was correlated with oxidative and inflammatory stress and the disease stages in CRF patients.
Identifiants
pubmed: 34529688
doi: 10.1371/journal.pone.0257176
pii: PONE-D-21-19910
pmc: PMC8445414
doi:
Substances chimiques
SHC1 protein, human
0
Src Homology 2 Domain-Containing, Transforming Protein 1
0
Malondialdehyde
4Y8F71G49Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0257176Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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