Dietary suppression of MHC class II expression in intestinal epithelial cells enhances intestinal tumorigenesis.

MHC-II antigen presentation cancer diet high-fat diet intestinal stem cells microbiome obesity

Journal

Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472

Informations de publication

Date de publication:
04 11 2021
Historique:
received: 19 10 2020
revised: 25 05 2021
accepted: 10 08 2021
pubmed: 17 9 2021
medline: 16 11 2021
entrez: 16 9 2021
Statut: ppublish

Résumé

Little is known about how interactions of diet, intestinal stem cells (ISCs), and immune cells affect early-stage intestinal tumorigenesis. We show that a high-fat diet (HFD) reduces the expression of the major histocompatibility complex class II (MHC class II) genes in intestinal epithelial cells, including ISCs. This decline in epithelial MHC class II expression in a HFD correlates with reduced intestinal microbiome diversity. Microbial community transfer experiments suggest that epithelial MHC class II expression is regulated by intestinal flora. Mechanistically, pattern recognition receptor (PRR) and interferon-gamma (IFNγ) signaling regulates epithelial MHC class II expression. MHC class II-negative (MHC-II-) ISCs exhibit greater tumor-initiating capacity than their MHC class II-positive (MHC-II+) counterparts upon loss of the tumor suppressor Apc coupled with a HFD, suggesting a role for epithelial MHC class II-mediated immune surveillance in suppressing tumorigenesis. ISC-specific genetic ablation of MHC class II increases tumor burden cell autonomously. Thus, HFD perturbs a microbiome-stem cell-immune cell interaction that contributes to tumor initiation in the intestine.

Identifiants

pubmed: 34529935
pii: S1934-5909(21)00344-1
doi: 10.1016/j.stem.2021.08.007
pmc: PMC8650761
mid: NIHMS1742234
pii:
doi:

Substances chimiques

Histocompatibility Antigens Class II 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1922-1935.e5

Subventions

Organisme : NCI NIH HHS
ID : R01 CA245314
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK043351
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK126545
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA014051
Pays : United States
Organisme : NCI NIH HHS
ID : F32 CA254314
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA250554
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA045508
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA014236
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA211184
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

Commentaires et corrections

Type : CommentIn
Type : CommentIn

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing financial interests. S.H.O. serves as an advisory board member for Cell Stem Cell. S.B. received research funding from Elstar Therapeutics and Revitope Oncology for research that is not related to this study.

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Auteurs

Semir Beyaz (S)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA; Division of Hematology/Oncology, Boston Children's Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA; The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA. Electronic address: beyaz@cshl.edu.

Charlie Chung (C)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Haiwei Mou (H)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Khristian E Bauer-Rowe (KE)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA.

Michael E Xifaras (ME)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA; The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA.

Ilgin Ergin (I)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Lenka Dohnalova (L)

Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Moshe Biton (M)

Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; The Department of Biological Regulation, Weizmann Institute of Science, Rehovot 7610001, Israel.

Karthik Shekhar (K)

Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Department of Chemical and Biomolecular Engineering, Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA.

Onur Eskiocak (O)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Katherine Papciak (K)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Kadir Ozler (K)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Mohammad Almeqdadi (M)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA.

Brian Yueh (B)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Miriam Fein (M)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Damodaran Annamalai (D)

Division of Comparative Medicine, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Eider Valle-Encinas (E)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA.

Aysegul Erdemir (A)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA.

Karoline Dogum (K)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA.

Vyom Shah (V)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Aybuke Alici-Garipcan (A)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Hannah V Meyer (HV)

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Deniz M Özata (DM)

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Eran Elinav (E)

Department of Immunology, Weizmann Institute of Science, Rehovot 7610001, Israel.

Alper Kucukural (A)

Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Pawan Kumar (P)

Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA.

Jeremy P McAleer (JP)

Department of Pharmaceutical Science and Research, Marshall University School of Pharmacy, Huntington, WV 25701, USA.

James G Fox (JG)

Division of Comparative Medicine, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Christoph A Thaiss (CA)

Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Aviv Regev (A)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02140, USA.

Jatin Roper (J)

Department of Medicine, Division of Gastroenterology, Duke University, Durham, NC 27710, USA.

Stuart H Orkin (SH)

Division of Hematology/Oncology, Boston Children's Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA. Electronic address: stuart_orkin@dfci.harvard.edu.

Ömer H Yilmaz (ÖH)

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA; Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. Electronic address: ohyilmaz@mit.edu.

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