Functional and structural alternations in the choroid plexus upon methamphetamine exposure.
Animals
Apoptosis
/ drug effects
Autophagy
/ drug effects
Brain-Derived Neurotrophic Factor
/ analysis
Caspase 3
/ analysis
Central Nervous System Stimulants
/ administration & dosage
Choroid Plexus
/ cytology
Epithelial Cells
/ drug effects
Injections, Intraperitoneal
Male
Methamphetamine
/ administration & dosage
Microscopy, Electron, Transmission
Rats
Up-Regulation
/ drug effects
Vascular Endothelial Growth Factor A
/ analysis
Autophagy
Choroid plexus
Differential gene expression
Methamphetamine
RNA-seq
Journal
Neuroscience letters
ISSN: 1872-7972
Titre abrégé: Neurosci Lett
Pays: Ireland
ID NLM: 7600130
Informations de publication
Date de publication:
01 11 2021
01 11 2021
Historique:
received:
24
07
2021
revised:
27
08
2021
accepted:
09
09
2021
pubmed:
17
9
2021
medline:
1
3
2022
entrez:
16
9
2021
Statut:
ppublish
Résumé
Choroid plexus (CP) is the principal source of cerebrospinal fluid. CP can produce and release a wide range of materials including growth factors, neurotrophic factors, etc. all of which play an important role in the maintenance and proper functioning of the brain. Methamphetamine (METH) is a CNS neurostimulant that causes brain dysfunction. Herein, we investigated the potential effects of METH exposure on CP structure and function. Stereological analysis revealed a significant alteration in CP volume, epithelial cells and capillary number upon METH treatment. Electron microscopy exhibited changes in ultrastructure. Moreover, the upregulation of neurotrophic factors such as BDNF and VEGF as well as autophagy and apoptosis gene following METH administration were observed. We also identified several signaling cascades related to autophagy. In conclusion, gene expression changes coupled with structural alterations of the CP in response to METH suggested METH-induced autophagy in CP.
Identifiants
pubmed: 34530114
pii: S0304-3940(21)00625-X
doi: 10.1016/j.neulet.2021.136246
pii:
doi:
Substances chimiques
Bdnf protein, rat
0
Brain-Derived Neurotrophic Factor
0
Central Nervous System Stimulants
0
Vascular Endothelial Growth Factor A
0
vascular endothelial growth factor A, rat
0
Methamphetamine
44RAL3456C
Casp3 protein, rat
EC 3.4.22.-
Caspase 3
EC 3.4.22.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
136246Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.