Using cyclodextrin-induced lipid substitution to study membrane lipid and ordered membrane domain (raft) function in cells.

Lipid Metabolism / genetics Membrane Lipids / chemistry Membrane Microdomains / chemistry Membrane Proteins / chemistry Mutation, Missense / genetics Phospholipids / chemistry alpha-Cyclodextrins / chemistry
Cyclodextrin Lipid asymmetry Phospholipid Plasma membrane Sphingolipid

Journal

Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713

Informations de publication

Date de publication:
01 02 2022
Historique:
received: 23 06 2021
revised: 30 08 2021
accepted: 08 09 2021
pubmed: 18 9 2021
medline: 29 12 2021
entrez: 17 9 2021
Statut: ppublish

Résumé

Methods for efficient cyclodextrin-induced lipid exchange have been developed in our lab. These make it possible to almost completely replace the lipids in the outer leaflet of artificial membranes or the plasma membranes of living cells with exogenous lipids. Lipid replacement/substitution allows detailed studies of how lipid composition and asymmetry influence the structure and function of membrane domains and membrane proteins. In this review, we both summarize progress on cyclodextrin exchange in cells, mainly by the use of methyl-alpha cyclodextrin to exchange phospholipids and sphingolipids, and discuss the issues to consider when carrying out lipid exchange experiments upon cells. Issues that impact interpretation of lipid exchange are also discussed. This includes how overly naïve interpretation of how lipid exchange-induced changes in domain formation can impact protein function.

Identifiants

DOI: 10.1016/j.bbamem.2021.183774 PMID: 34534531 PMC: PMC9128603
pubmed: 34534531
pii: S0005-2736(21)00222-4
doi: 10.1016/j.bbamem.2021.183774
pmc: PMC9128603
mid: NIHMS1804436
pii:
doi:

Substances chimiques

Membrane Lipids 0
Membrane Proteins 0
Phospholipids 0
alpha-Cyclodextrins 0
alpha-cyclodextrin Z1LH97KTRM

Types de publication

Journal Article Research Support, N.I.H., Extramural Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

183774

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM122493
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

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Auteurs

Pavana Suresh (P)

Dept. of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA.

Erwin London (E)

Dept. of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA. Electronic address: Erwin.London@stonybrook.edu.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Métabolisme lipidique › Using cyclodextrin-induced lipid substitution...
questionsmedicales.fr › Lipides › Lipides membranaires › Using cyclodextrin-induced lipid substitution...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Microdomaines membranaires › Using cyclodextrin-induced lipid substitution...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Using cyclodextrin-induced lipid substitution...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Mutation faux-sens › Using cyclodextrin-induced lipid substitution...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Using cyclodextrin-induced lipid substitution...
questionsmedicales.fr › Glucides › Polyosides › Glucanes › Amidon › Dextrine › Cyclodextrines › Cyclodextrines alpha › Using cyclodextrin-induced lipid substitution...