Reaction dynamics and residue identification of haemoglobin modification by acrolein, a lipid-peroxidation by-product.

Acrolein Aldehydes Lipid Peroxidation
Acrolein Biomarker Diabetes Haemoglobin Liquid chromatography-mass spectrometry (LC-MS/MS) Oxidative stress

Journal

Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726

Informations de publication

Date de publication:
12 2021
Historique:
received: 14 05 2021
revised: 06 09 2021
accepted: 10 09 2021
pubmed: 18 9 2021
medline: 24 12 2021
entrez: 17 9 2021
Statut: ppublish

Résumé

Lipid hydroperoxides decompose to reactive aldehydes, such as acrolein. Measurement of oxidative stress markers in the clinic could improve risk stratification for patients. To aid the development of diagnostic oxidative stress markers, we defined the acrolein modifications of haemoglobin using mass spectrometry. Acrolein modifications have little effect on the secondary structure of haemoglobin. They do not disrupt the quaternary structure, but instead promote crosslinked octamers. For acrolein modified haemoglobin the response to O We have generated qualitative evidence identifying the acrolein target sites on haemoglobin, a potential oxidative stress marker that is easily measured in circulation. We provide data for the community to develop targeted mass spectrometric or immunometric assays for acrolein modified haemoglobin to further validate the potential of haemoglobin as an oxidative stress marker in patients .

Sections du résumé

BACKGROUND
Lipid hydroperoxides decompose to reactive aldehydes, such as acrolein. Measurement of oxidative stress markers in the clinic could improve risk stratification for patients.
METHODS
To aid the development of diagnostic oxidative stress markers, we defined the acrolein modifications of haemoglobin using mass spectrometry.
RESULTS
Acrolein modifications have little effect on the secondary structure of haemoglobin. They do not disrupt the quaternary structure, but instead promote crosslinked octamers. For acrolein modified haemoglobin the response to O
CONCLUSIONS
We have generated qualitative evidence identifying the acrolein target sites on haemoglobin, a potential oxidative stress marker that is easily measured in circulation.
GENERAL SIGNIFICANCE
We provide data for the community to develop targeted mass spectrometric or immunometric assays for acrolein modified haemoglobin to further validate the potential of haemoglobin as an oxidative stress marker in patients .

Identifiants

DOI: 10.1016/j.bbagen.2021.130013 PMID: 34534644
pubmed: 34534644
pii: S0304-4165(21)00172-0
doi: 10.1016/j.bbagen.2021.130013
pii:
doi:

Substances chimiques

Aldehydes 0
Acrolein 7864XYD3JJ

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

130013

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Moritz Lassé (M)

Christchurch Heart Institute, Department of Medicine, University of Otago, Christchurch, New Zealand.

Anja R Stampfli (AR)

Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.

Thomas Orban (T)

Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.

Roshit K Bothara (RK)

Canterbury District Health Board, Christchurch, New Zealand.

Juliet A Gerrard (JA)

Faculty of Science, Biological Sciences, The University of Auckland, Auckland, New Zealand.

Antony J Fairbanks (AJ)

Biomolecular Interaction Centre, and School of Physical and Chemical Sciences, University of Canterbury, Christchurch, New Zealand.

Neil R Pattinson (NR)

Canterbury Scientific Ltd, 71 Whiteleigh Ave, Christchurch, New Zealand.

Renwick C J Dobson (RCJ)

Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; Biol21 Molecular Science and Biotechnology Institute, Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: renwick.dobson@canterbury.ac.nz.

Articles similaires

Saccharomyces cerevisiae whole cell biotransformation for the production of aldehyde flavors and fragrances.

Nazreen V M Abdul Muthaliff, Yao Zong Ng, Wei Mei Guo et al.
1.00
Saccharomyces cerevisiae Aldehydes Biotransformation Flavoring Agents Lipoxygenase

Investigation of the impact of diverse climate conditions on the cultivation suitability of Cinnamomum cassia using the MaxEnt model, HPLC and chemometric methods in China.

Yanqun Li, Yang Yang, Dingze Gu et al.
1.00
Cinnamomum aromaticum China Chromatography, High Pressure Liquid Ecosystem Climate Change

The link between amyloid β and ferroptosis pathway in Alzheimer's disease progression.

Naďa Majerníková, Alejandro Marmolejo-Garza, Casandra Salinas Salinas et al.
1.00
Ferroptosis Humans Alzheimer Disease Amyloid beta-Peptides Disease Progression

Is castration leading to biological aging in dogs? Assessment of lipid peroxidation, inflammation, telomere length, mitochondrial DNA copy number, and expression of telomerase and age-related genes.

Hossein Hassanpour, Moosa Javdani, Zahra Changaniyan-Khorasgani et al.
1.00
Animals Dogs Male Lipid Peroxidation Aging

Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Aldéhydes › Acroléine › Reaction dynamics and residue identification...
questionsmedicales.fr › Composés chimiques organiques › Aldéhydes › Reaction dynamics and residue identification...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Peroxydation lipidique › Reaction dynamics and residue identification...