Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors.
Anisakis simplex
Cytokine
Dendritic Cell
Flow cytometry
Immunoregulation
Innate Immunity
Journal
International immunopharmacology
ISSN: 1878-1705
Titre abrégé: Int Immunopharmacol
Pays: Netherlands
ID NLM: 100965259
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
02
04
2021
revised:
30
08
2021
accepted:
30
08
2021
pubmed:
20
9
2021
medline:
22
2
2022
entrez:
19
9
2021
Statut:
ppublish
Résumé
The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs). In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Moreover, when A. simplex larval antigens were combined with TLR agonists (TLR 1/2-9), they modified mainly TLR2, TLR4 and TLR9 agonists responses in both mice strains, and also TLR3, TLR5 and TLR7 in BALB/c. Antigen-presenting ability was analyzed by the detection of CD11c + cells expressing surface markers (CD80-86, MHC I-II), intracellular cytokines (IL-10, IL-12, TNF-α) and intracellular proteins (Myd88, NF-κβ) by Flow Cytometry. Secreted IL-10 was measured by ELISA. Our findings confirm not only that the host genetic basis plays a role in the development of a Th2/Th1/T
Identifiants
pubmed: 34537480
pii: S1567-5769(21)00756-6
doi: 10.1016/j.intimp.2021.108120
pii:
doi:
Substances chimiques
Antigens
0
H-2 Antigens
0
H-2K(sm1) antigen
0
Toll-Like Receptor 2
0
Toll-Like Receptors
0
Interleukin-10
130068-27-8
Interleukin-12
187348-17-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108120Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.