Ablation of mitochondrial DNA results in widespread remodeling of the mitochondrial complexome.
Adaptation, Physiological
Adenosine Triphosphate
/ metabolism
Cell Line, Tumor
DNA, Mitochondrial
/ genetics
Gene Expression
Humans
Mitochondria
/ metabolism
Mitochondrial Membranes
/ chemistry
Mitochondrial Proteins
/ deficiency
Multiprotein Complexes
/ deficiency
Osteoblasts
/ metabolism
Oxidative Phosphorylation
OXPHOS
complexome profiling
mitochondria
mtDNA
rho0 cells
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
02 11 2021
02 11 2021
Historique:
revised:
26
08
2021
received:
05
05
2021
accepted:
01
09
2021
pubmed:
21
9
2021
medline:
15
12
2021
entrez:
20
9
2021
Statut:
ppublish
Résumé
So-called ρ0 cells lack mitochondrial DNA and are therefore incapable of aerobic ATP synthesis. How cells adapt to survive ablation of oxidative phosphorylation remains poorly understood. Complexome profiling analysis of ρ0 cells covered 1,002 mitochondrial proteins and revealed changes in abundance and organization of numerous multiprotein complexes including previously not described assemblies. Beyond multiple subassemblies of complexes that would normally contain components encoded by mitochondrial DNA, we observed widespread reorganization of the complexome. This included distinct changes in the expression pattern of adenine nucleotide carrier isoforms, other mitochondrial transporters, and components of the protein import machinery. Remarkably, ablation of mitochondrial DNA hardly affected the complexes organizing cristae junctions indicating that the altered cristae morphology in ρ0 mitochondria predominantly resulted from the loss of complex V dimers required to impose narrow curvatures to the inner membrane. Our data provide a comprehensive resource for in-depth analysis of remodeling of the mitochondrial complexome in response to respiratory deficiency.
Identifiants
pubmed: 34542926
doi: 10.15252/embj.2021108648
pmc: PMC8561636
doi:
Substances chimiques
DNA, Mitochondrial
0
Mitochondrial Proteins
0
Multiprotein Complexes
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e108648Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
Références
Cell Metab. 2014 Aug 5;20(2):241-52
pubmed: 24954416
Diabetes. 2014 May;63(5):1488-505
pubmed: 24379352
PLoS One. 2014 Jun 09;9(6):e99270
pubmed: 24911211
EMBO J. 2003 Feb 17;22(4):816-25
pubmed: 12574118
Physiology (Bethesda). 2020 Sep 1;35(5):302-327
pubmed: 32783608
Hum Mol Genet. 2013 Apr 1;22(7):1348-57
pubmed: 23263864
Biochim Biophys Acta Bioenerg. 2021 Jul 1;1862(7):148411
pubmed: 33722514
Sci Rep. 2017 Nov 1;7(1):14864
pubmed: 29093569
Mol Biol Cell. 2014 Nov 1;25(21):3342-9
pubmed: 25165143
Nature. 1994 Oct 27;371(6500):768-74
pubmed: 7935837
Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):1004-11
pubmed: 20188060
J Cell Biol. 2019 Feb 4;218(2):598-614
pubmed: 30598479
EMBO J. 2020 Jul 15;39(14):e103912
pubmed: 32511785
Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):2988-2993
pubmed: 29440398
Bioinformatics. 2004 Jun 12;20(9):1453-4
pubmed: 14871861
Nat Cell Biol. 2018 Jul;20(7):745-754
pubmed: 29950572
J Biol Chem. 2008 Sep 26;283(39):26312-23
pubmed: 18667415
Cell Metab. 2012 Oct 3;16(4):538-49
pubmed: 22982022
FEBS Lett. 2016 Dec;590(23):4147-4158
pubmed: 27718247
J Cell Biol. 2005 Sep 26;170(7):1067-78
pubmed: 16172208
Mol Cell. 2017 Aug 3;67(3):457-470.e5
pubmed: 28712726
Nat Rev Mol Cell Biol. 2020 Apr;21(4):204-224
pubmed: 32071438
FEBS Lett. 2007 Jul 24;581(18):3545-9
pubmed: 17624330
Science. 2009 Aug 28;325(5944):1139-42
pubmed: 19628817
Biochim Biophys Acta. 2012 Feb;1823(2):348-57
pubmed: 22178133
Cell Metab. 2014 Aug 5;20(2):253-66
pubmed: 24954417
Mol Cell. 2017 Aug 3;67(3):471-483.e7
pubmed: 28712724
Oncogene. 2011 Feb 24;30(8):883-95
pubmed: 21076465
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Cell Metab. 2017 Jan 10;25(1):57-71
pubmed: 28094012
Proc Natl Acad Sci U S A. 2014 Oct 14;111(41):14782-7
pubmed: 25275009
EMBO J. 2004 Jun 16;23(12):2423-9
pubmed: 15167897
J Bioenerg Biomembr. 2012 Jun;44(3):333-40
pubmed: 22555559
Mol Cell Proteomics. 2010 Oct;9(10):2149-61
pubmed: 20173216
Science. 2015 Apr 3;348(6230):95-98
pubmed: 25838379
Nature. 2020 Dec;588(7836):174-179
pubmed: 32906142
Mol Cell Biol. 2009 Feb;29(4):1007-16
pubmed: 19075006
Lancet. 2018 Jun 23;391(10139):2560-2574
pubmed: 29903433
J Biol Chem. 2002 Nov 1;277(44):41455-62
pubmed: 12194972
FEBS Lett. 1986 Aug 11;204(1):9-15
pubmed: 3017752
Mol Cell Proteomics. 2005 Dec;4(12):2010-21
pubmed: 16249172
PLoS One. 2013;8(1):e53774
pubmed: 23341998
Biochim Biophys Acta. 2016 Jan;1863(1):91-101
pubmed: 26477565
Mol Biol Cell. 2010 Apr 15;21(8):1315-23
pubmed: 20200222
EMBO J. 2021 Jan 15;40(2):e105513
pubmed: 33197065
EMBO J. 2003 Oct 1;22(19):4945-56
pubmed: 14517234
Bioinformatics. 2015 Feb 1;31(3):440-1
pubmed: 25301849
Science. 1989 Oct 27;246(4929):500-3
pubmed: 2814477
J Cell Biol. 2014 Mar 31;204(7):1083-6
pubmed: 24687277
Biochim Biophys Acta Bioenerg. 2021 Jun 1;1862(6):148395
pubmed: 33600785
Nucleic Acids Res. 2016 Jan 4;44(D1):D1251-7
pubmed: 26450961
PLoS Genet. 2013;9(12):e1004034
pubmed: 24385928
ChemMedChem. 2016 Apr 19;11(8):738-56
pubmed: 26864455
J Cell Physiol. 2004 Dec;201(3):470-82
pubmed: 15389597
Nucleic Acids Res. 2015 Apr 30;43(8):4284-95
pubmed: 25824949
Science. 2012 Jul 6;337(6090):96-100
pubmed: 22628558
Ann Neurol. 2006 Feb;59(2):265-75
pubmed: 16365880
Eur J Biochem. 1999 May;262(1):108-16
pubmed: 10231371
Bioinformatics. 2019 Sep 1;35(17):3083-3091
pubmed: 30649188
Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14121-6
pubmed: 21836051
J Neurochem. 2017 Nov;143(4):418-431
pubmed: 28397282
Cell Metab. 2014 Jul 1;20(1):158-71
pubmed: 24856930
Cell Rep. 2017 Feb 14;18(7):1727-1738
pubmed: 28199844
Ann Neurol. 2001 Jan;49(1):106-10
pubmed: 11198278
EMBO J. 2006 Jul 12;25(13):2966-77
pubmed: 16778770
Cell Metab. 2020 Sep 1;32(3):479-497.e9
pubmed: 32877691
Mol Biol Cell. 2002 Jan;13(1):71-83
pubmed: 11809823
J Biol Chem. 1998 Sep 4;273(36):22983-9
pubmed: 9722521
Annu Rev Biochem. 2017 Jun 20;86:685-714
pubmed: 28301740
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23519-23526
pubmed: 32900941
Cell Metab. 2017 Jan 10;25(1):128-139
pubmed: 27720676
Nat Protoc. 2006;1(1):418-28
pubmed: 17406264
Mol Biol Cell. 2012 Apr;23(7):1157-66
pubmed: 22323289
Curr Biol. 2006 Nov 21;16(22):2271-6
pubmed: 17113393