Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
Staphylococcus aureus
heparan sulfate
intravital microscopy
liver
neutrophils
proteomics
sepsis
thrombosis
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
26 10 2021
26 10 2021
Historique:
pubmed:
22
9
2021
medline:
20
1
2022
entrez:
21
9
2021
Statut:
ppublish
Résumé
Hepatic failure is an important risk factor for poor outcome in septic patients. Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis. These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors. Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection. Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
Identifiants
pubmed: 34544271
doi: 10.1128/mBio.01181-21
pmc: PMC8546592
doi:
Substances chimiques
Heparitin Sulfate
9050-30-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0118121Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008806
Pays : United States
Organisme : NINDS NIH HHS
ID : P30 NS047101
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM119850
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL131474
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL078784
Pays : United States
Références
Cell Host Microbe. 2018 Aug 8;24(2):271-284.e3
pubmed: 30033122
J Clin Invest. 2019 Apr 1;129(4):1779-1784
pubmed: 30720464
Nat Rev Immunol. 2013 Jan;13(1):34-45
pubmed: 23222502
Blood. 2012 Aug 23;120(8):1742-51
pubmed: 22791291
Science. 2013 Jan 18;339(6117):328-32
pubmed: 23329049
J Leukoc Biol. 2018 Aug;104(2):413-422
pubmed: 29733455
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D655-8
pubmed: 16381952
Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15880-5
pubmed: 20798043
J Biol Chem. 2015 Aug 28;290(35):21292-304
pubmed: 26183778
Prog Mol Biol Transl Sci. 2010;93:179-212
pubmed: 20807646
Proc Natl Acad Sci U S A. 2019 Nov 19;116(47):23618-23624
pubmed: 31712416
Trends Mol Med. 2014 Apr;20(4):195-203
pubmed: 24581450
Pflugers Arch. 2007 Jun;454(3):345-59
pubmed: 17256154
Nat Immunol. 2005 Sep;6(9):902-10
pubmed: 16056228
Nat Med. 2012 Aug;18(8):1217-23
pubmed: 22820644
J Clin Invest. 2001 Jul;108(2):175-80
pubmed: 11457868
J Exp Med. 2008 Apr 14;205(4):915-27
pubmed: 18362172
Cell Host Microbe. 2015 Jun 10;17(6):775-87
pubmed: 26067604
JAMA. 2016 Feb 23;315(8):801-10
pubmed: 26903338
J Thromb Haemost. 2019 Feb;17(2):283-294
pubmed: 30582882
Genesis. 2010 Sep;48(9):563-7
pubmed: 20645309
J Leukoc Biol. 2015 Oct;98(4):503-15
pubmed: 25979432
Infect Immun. 2015 Sep;83(9):3648-56
pubmed: 26150541
Blood. 2010 Sep 16;116(11):1924-31
pubmed: 20530797
Sci Transl Med. 2020 Mar 18;12(535):
pubmed: 32188725
J Immunol. 2006 Nov 15;177(10):6991-8
pubmed: 17082614
J Exp Med. 2016 Jun 27;213(7):1141-51
pubmed: 27325887
Nat Rev Nephrol. 2018 Jul;14(7):417-427
pubmed: 29691495
J Surg Res. 2011 Jan;165(1):136-41
pubmed: 19560161
J Exp Med. 2012 Apr 9;209(4):819-35
pubmed: 22451716
Am J Respir Cell Mol Biol. 2017 Jun;56(6):727-737
pubmed: 28187268
Nat Rev Immunol. 2017 Jul;17(7):407-420
pubmed: 28436424
Cell Host Microbe. 2016 Jul 13;20(1):99-106
pubmed: 27345697
Pediatr Infect Dis J. 2006 Apr;25(4):349-53
pubmed: 16567988
Nat Commun. 2016 Jan 06;7:10261
pubmed: 26732734
Dev Biol. 2001 Feb 15;230(2):230-42
pubmed: 11161575
Circ Res. 1996 Sep;79(3):581-9
pubmed: 8781491
Cell. 2017 Nov 30;171(6):1368-1382.e23
pubmed: 29195076
Curr Biol. 2012 Dec 18;22(24):2375-82
pubmed: 23219724
Nat Methods. 2011 Jan;8(1):91-6
pubmed: 21151136
Vascul Pharmacol. 2018 Jan;100:26-33
pubmed: 28919014
J Immunol. 2013 Jul 1;191(1):448-55
pubmed: 23733868
Immunity. 2010 Nov 24;33(5):817-29
pubmed: 21093315
Crit Care. 2019 Jan 17;23(1):16
pubmed: 30654825
Nature. 2007 Apr 26;446(7139):1030-7
pubmed: 17460664
Science. 2010 Oct 15;330(6002):362-6
pubmed: 20947763
Development. 2005 Aug;132(16):3777-86
pubmed: 16020517
J Cell Biol. 2007 May 7;177(3):539-49
pubmed: 17470635
Cell. 1997 Oct 31;91(3):385-95
pubmed: 9363947
Nat Methods. 2020 Jan;17(1):41-44
pubmed: 31768060
Nat Commun. 2015 Mar 26;6:6673
pubmed: 25809117
J Biol Chem. 2000 Aug 25;275(34):25926-30
pubmed: 10852901
Mol Cell Proteomics. 2017 Dec;16(12):2296-2309
pubmed: 29070702
Nature. 2018 Oct;562(7727):367-372
pubmed: 30283141
Crit Rev Immunol. 2015;35(4):277-91
pubmed: 26757392
Nat Commun. 2019 Oct 11;10(1):4656
pubmed: 31604940
Front Immunol. 2017 May 10;8:530
pubmed: 28539925
Sci Data. 2020 Mar 26;7(1):104
pubmed: 32218446