Engineering T4 Bacteriophage for

Bacteriophage T4 / genetics CRISPR-Cas Systems Cell Surface Display Techniques Escherichia coli / genetics Gene Editing / methods
T4 bacteriophage genome engineering phage display type V CRISPR

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
15 10 2021
Historique:
pubmed: 22 9 2021
medline: 3 3 2022
entrez: 21 9 2021
Statut: ppublish

Résumé

Bacteriophage T4 has enormous potential for biomedical applications due to its large size, capsid architecture, and high payload capability for protein and DNA delivery. However, it is not very easy to genetically engineer its genome heavily modified by cytosine hydroxymethylation and glucosylation. The glucosyl hydroxymethyl cytosine (ghmC) genome of phage is completely resistant to most restriction endonucleases and exhibits various degrees of resistance to CRISPR-Cas systems. Here, we found that the type V CRISPR-Cas12a system, which shows efficient cleavage of ghmC-modified genome when compared to the type II CRISPR-Cas9 system, can be synergistically employed to generate recombinant T4 phages. Focused on surface display, we analyzed the ability of phage T4 outer capsid proteins Hoc (highly antigenic outer capsid protein) and Soc (small outer capsid protein) to tether,

Identifiants

DOI: 10.1021/acssynbio.1c00251 PMID: 34546037
pubmed: 34546037
doi: 10.1021/acssynbio.1c00251
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

2639-2648

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI081726
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI111538
Pays : United States

Auteurs

Junhua Dong (J)

Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Hongshan Laboratory, Wuhan 430070, Hubei, China.

Cen Chen (C)

Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Hongshan Laboratory, Wuhan 430070, Hubei, China.

Yuepeng Liu (Y)

Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Hongshan Laboratory, Wuhan 430070, Hubei, China.

Jingen Zhu (J)

Bacteriophage Medical Research Center, Department of Biology, The Catholic University of America, Washington, District of Columbia 20064, United States.

Mengling Li (M)

Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Hongshan Laboratory, Wuhan 430070, Hubei, China.

Venigalla B Rao (VB)

Bacteriophage Medical Research Center, Department of Biology, The Catholic University of America, Washington, District of Columbia 20064, United States.

Pan Tao (P)

Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Hongshan Laboratory, Wuhan 430070, Hubei, China.
ORCID: 0000-0002-0082-2229

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Classifications MeSH

questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Myoviridae › Bactériophage T4 › Engineering T4 Bacteriophage for
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Engineering T4 Bacteriophage for
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Techniques d'exposition à la surface cellulaire › Engineering T4 Bacteriophage for
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Engineering T4 Bacteriophage for
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Engineering T4 Bacteriophage for