TRAIL signaling promotes entosis in colorectal cancer.
Apoptosis
/ physiology
Apoptosis Regulatory Proteins
/ metabolism
Caspase 8
/ metabolism
Caspases
/ metabolism
Cell Death
/ physiology
Cell Line, Tumor
Colonic Neoplasms
/ metabolism
Entosis
/ physiology
HCT116 Cells
Humans
Membrane Glycoproteins
/ metabolism
Receptors, TNF-Related Apoptosis-Inducing Ligand
/ metabolism
Signal Transduction
/ physiology
TNF-Related Apoptosis-Inducing Ligand
/ metabolism
Tumor Necrosis Factor-alpha
/ metabolism
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 11 2021
01 11 2021
Historique:
received:
07
10
2020
revised:
14
07
2021
accepted:
31
08
2021
entrez:
21
9
2021
pubmed:
22
9
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Entosis is a form of nonphagocytic cell-in-cell (CIC) interaction where a living cell enters into another. Tumors show evidence of entosis; however, factors controlling entosis remain to be elucidated. Here, we find that besides inducing apoptosis, TRAIL signaling is a potent activator of entosis in colon cancer cells. Initiation of both apoptosis and entosis requires TRAIL receptors DR4 and DR5; however, induction of apoptosis and entosis diverges at caspase-8 as its structural presence is sufficient for induction of entosis but not apoptosis. Although apoptosis and entosis are morphologically and biochemically distinct, knockout of Bax and Bak, or inhibition of caspases, also inhibits entotic cell death and promotes survival and release of inner cells. Analysis of colorectal cancer tumors reveals a significant association between TRAIL signaling and CIC structures. Finally, the presence of CIC structures in the invasive front regions of colorectal tumors shows a strong correlation with adverse patient prognosis.
Identifiants
pubmed: 34546352
pii: 212649
doi: 10.1083/jcb.202010030
pmc: PMC8563286
pii:
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
Membrane Glycoproteins
0
Receptors, TNF-Related Apoptosis-Inducing Ligand
0
TNF-Related Apoptosis-Inducing Ligand
0
TNFSF10 protein, human
0
Tumor Necrosis Factor-alpha
0
Caspase 8
EC 3.4.22.-
Caspases
EC 3.4.22.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical Research Council
ID : MR/S021205/1
Pays : United Kingdom
Organisme : Cancer Research UK
ID : 24387
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T002824/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0400302
Pays : United Kingdom
Organisme : Health Research Board
ID : 16/US/330
Pays : Ireland
Organisme : Science Foundation Ireland
ID : 14/IA/2582
Pays : Ireland
Organisme : Wellcome Trust
ID : 110371/Z/15/Z
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 Bozkurt et al.
Références
Front Immunol. 2019 Jul 03;10:1530
pubmed: 31333662
Nat Cell Biol. 2011 Oct 16;13(11):1335-43
pubmed: 22002674
Clin Cancer Res. 2010 Jul 1;16(13):3442-51
pubmed: 20570920
Sci Rep. 2017 Oct 2;7(1):12555
pubmed: 28970591
Mol Cell. 2017 Feb 16;65(4):730-742.e5
pubmed: 28212753
J Biol Chem. 2008 Aug 1;283(31):21676-85
pubmed: 18522940
Mol Syst Biol. 2015 May 07;11(5):803
pubmed: 25953765
Proc Natl Acad Sci U S A. 2017 Jan 17;114(3):504-509
pubmed: 28049841
Phys Biol. 2013 Jun;10(3):035002
pubmed: 23735516
Nat Commun. 2018 Aug 3;9(1):3070
pubmed: 30076358
Radiat Oncol. 2017 Jan 18;12(1):21
pubmed: 28100275
Int J Biochem Cell Biol. 2007;39(7-8):1462-75
pubmed: 17403612
J Biol Chem. 2000 Aug 11;275(32):24357-66
pubmed: 10823821
Mol Biol Cell. 2012 Jun;23(11):2076-91
pubmed: 22496418
Nat Methods. 2010 Jun;7(6):418-9
pubmed: 20508634
World J Gastroenterol. 2009 Dec 21;15(47):5898-906
pubmed: 20014453
Cold Spring Harb Perspect Biol. 2013 May 01;5(5):a008698
pubmed: 23637280
Cell Mol Life Sci. 2016 Jun;73(11-12):2379-86
pubmed: 27048820
Oncogene. 2012 Jun 28;31(26):3177-89
pubmed: 22056880
Nat Rev Mol Cell Biol. 2008 Oct;9(10):796-809
pubmed: 18784728
Cell Rep. 2017 Jul 5;20(1):201-210
pubmed: 28683313
Hum Pathol. 2016 Jan;47(1):4-19
pubmed: 26476568
Gastroenterology. 2009 Jul;137(1):188-98, 198.e1-2
pubmed: 19361506
Mol Cell. 2017 Feb 16;65(4):715-729.e5
pubmed: 28212752
J Cell Biol. 2019 Nov 4;218(11):3827-3844
pubmed: 31530580
Cell Death Discov. 2016 Oct 03;2:16065
pubmed: 27752362
Cancers (Basel). 2019 Sep 30;11(10):
pubmed: 31574961
Sci Rep. 2017 Dec 4;7(1):16878
pubmed: 29203879
Mod Pathol. 2017 Sep;30(9):1299-1311
pubmed: 28548122
Cell Res. 2014 Nov;24(11):1299-310
pubmed: 25342560
Cell Death Dis. 2013 Jul 04;4:e702
pubmed: 23828565
Cell Death Differ. 2013 Jul;20(7):845-6
pubmed: 23749177
Breast Cancer Res Treat. 2008 Feb;107(3):349-57
pubmed: 17453339
Dev Cell. 2018 Mar 12;44(5):555-565.e3
pubmed: 29533771
Cell. 2007 Nov 30;131(5):966-79
pubmed: 18045538
Cell Death Dis. 2018 Jun 18;9(7):716
pubmed: 29915308
Oncotarget. 2017 Feb 7;8(6):9974-9985
pubmed: 28039489
Mol Biol Cell. 2013 Jul;24(14):2186-200
pubmed: 23699397
Int J Mol Sci. 2018 May 23;19(6):
pubmed: 29789517
Cancer Immunol Immunother. 2005 May;54(5):499-505
pubmed: 15614529
Br J Cancer. 1972 Aug;26(4):239-57
pubmed: 4561027
Immunity. 1995 Dec;3(6):673-82
pubmed: 8777713
Elife. 2017 Jul 11;6:
pubmed: 28693721
Front Biosci. 2007 May 01;12:3813-24
pubmed: 17485341
Cell Death Differ. 2016 May;23(5):733-47
pubmed: 26943322