Escherichia coli Nissle 1917 secondary metabolism: aryl polyene biosynthesis and phosphopantetheinyl transferase crosstalk.
Aryl polyenes
Biosynthetic gene cluster
Colibactin
Escherichia coli Nissle 1917
Phosphopantetheinyl transferase crosstalk
Siderophores
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
03
06
2021
accepted:
24
08
2021
revised:
21
08
2021
pubmed:
22
9
2021
medline:
13
10
2021
entrez:
21
9
2021
Statut:
ppublish
Résumé
Escherichia coli Nissle 1917 (EcN) is a Gram-negative bacterium that is used to treat inflammatory bowel diseases. The probiotic character of EcN is not well-understood, but its ability to produce secondary metabolites plays an important role in its activity. The EcN genome encodes for an aryl polyene (APE) biosynthetic gene cluster (BGC), and APE products have a role in biofilm formation. We show here that this unusual polyketide assembly line synthase produces four APE molecules which are likely cis/trans isomers. Within the APE BGC, two acyl carrier proteins are involved in biosynthesis. Acyl carrier proteins require activation by post-translational modification with a phosphopantetheinyl transferase (PPTase). Through analysis of single, double, and triple mutants of three PPTases, the PPTase-BGC crosstalk relationship in EcN was characterized. Understanding PPTase-BGC crosstalk is important for the engineering of secondary metabolite production hosts and for targeting of PPTases with new antibiotics. KEY POINTS: • Escherichia coli Nissle 1917 biosynthesizes four aryl polyene isoforms. • Phosphopantetheinyl transferase crosstalk is important for biosynthesis.
Identifiants
pubmed: 34546406
doi: 10.1007/s00253-021-11546-x
pii: 10.1007/s00253-021-11546-x
doi:
Substances chimiques
Bacterial Proteins
0
Escherichia coli Proteins
0
Polyenes
0
phosphopantetheinyl transferase
0
Transferases (Other Substituted Phosphate Groups)
EC 2.7.8.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7785-7799Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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