Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
18
03
2021
accepted:
01
09
2021
entrez:
21
9
2021
pubmed:
22
9
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014-2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3'')-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans.
Identifiants
pubmed: 34547028
doi: 10.1371/journal.pone.0249617
pii: PONE-D-21-08953
pmc: PMC8454963
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0249617Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Vet Clin North Am Food Anim Pract. 2018 Mar;34(1):133-154
pubmed: 29224803
Emerg Infect Dis. 2017 Sep;23(9):
pubmed: 28820133
Bioinformatics. 2014 May 1;30(9):1312-3
pubmed: 24451623
Microb Drug Resist. 2019 Oct;25(8):1238-1249
pubmed: 31149890
Antimicrob Agents Chemother. 2009 Jun;53(6):2696-9
pubmed: 19332677
J Antimicrob Chemother. 2012 Nov;67(11):2640-4
pubmed: 22782487
Epidemiol Infect. 2016 Aug;144(11):2430-9
pubmed: 26996313
Microbiol Resour Announc. 2019 Apr 4;8(14):
pubmed: 30948462
BMC Microbiol. 2019 Oct 16;19(1):226
pubmed: 31619165
Front Microbiol. 2019 Apr 17;10:832
pubmed: 31057528
Antimicrob Agents Chemother. 2014 Jul;58(7):3895-903
pubmed: 24777092
J Antimicrob Chemother. 2018 Feb 1;73(2):365-372
pubmed: 29216342
J Glob Antimicrob Resist. 2019 Sep;18:179-182
pubmed: 30926467
J Bacteriol. 1997 Apr;179(7):2274-80
pubmed: 9079913
PLoS One. 2020 Jan 24;15(1):e0220274
pubmed: 31978098
Appl Environ Microbiol. 2017 May 31;83(12):
pubmed: 28389536
Front Microbiol. 2021 Jan 14;11:607842
pubmed: 33519755
Microbiol Resour Announc. 2019 Jan 3;8(1):
pubmed: 30637388
Mol Biol Evol. 2003 Apr;20(4):653-9
pubmed: 12679553
J Antimicrob Chemother. 2017 Oct 1;72(10):2764-2768
pubmed: 29091202
Antimicrob Agents Chemother. 2016 Aug 22;60(9):5515-20
pubmed: 27381390
Genes (Basel). 2020 Oct 30;11(11):
pubmed: 33142960
J Clin Microbiol. 1999 Aug;37(8):2418-27
pubmed: 10405378
Antimicrob Agents Chemother. 2019 May 24;63(6):
pubmed: 31036694
BMC Infect Dis. 2017 Aug 4;17(1):544
pubmed: 28778189
Microb Drug Resist. 2021 Jun;27(6):792-799
pubmed: 33232624
Antimicrob Agents Chemother. 2019 Oct 22;63(11):
pubmed: 31427293
Environ Microbiol. 2020 Jan;22(1):413-432
pubmed: 31715658
J Infect Public Health. 2020 Jan;13(1):94-100
pubmed: 31279801
One Health Outlook. 2020 Jul 6;2:13
pubmed: 33829134