Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region.

DNA Methylation DNA, Intergenic Female Humans Mouth Mucosa / metabolism Oxytocin / genetics Pregnancy Receptors, Oxytocin / genetics Vasopressins
DNA methylation Epigenetics Intergenic region OXTR Oxytocin Saliva

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 22 9 2021
pubmed: 23 9 2021
medline: 8 1 2022
Statut: ppublish

Résumé

Oxytocin and its paralogue, vasopressin, are widely studied biomarkers in relation to pregnancy and birth, maternal and social behavior, and mental health. Epigenetics is a biological mechanism that mediates the link between environmental influences and behavioral patterns. In a candidate gene approach, we describe here a DNA methylation assay of two regions within the oxytocin system, using human buccal cells and next-generation sequencing. Two nanograms of DNA were sufficient to assess the DNA methylation status of 28 CpG sites (22 corresponding to the oxytocin receptor and 6 corresponding to the vasopressin/oxytocin intergenic region). This method proved to be non-invasive for the participants, and reproducible; its validity remains to be confirmed alongside other biomarkers of gene function.

Identifiants

DOI: 10.1007/978-1-0716-1759-5_6 PMID: 34550570
pubmed: 34550570
doi: 10.1007/978-1-0716-1759-5_6
doi:

Substances chimiques

DNA, Intergenic 0
Receptors, Oxytocin 0
Vasopressins 11000-17-2
Oxytocin 50-56-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

81-103

Subventions

Organisme : CIHR
ID : MOP-123354
Pays : Canada

Informations de copyright

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Leonora King (L)

Jewish General Hospital, Lady Davis Institute for Medical Research and Department of Psychiatry, McGill University, Montreal, QC, Canada.

Stephanie Robins (S)

Institute of Community and Family Psychiatry, Jewish General Hospital and Lady Davis Institute for Medical Research, Montreal, QC, Canada.

Gary Chen (G)

McGill Group for Suicide Studies, Douglas Mental Health University Institute, Montreal, QC, Canada.

Gustavo Turecki (G)

McGill Group for Suicide Studies, Douglas Mental Health University Institute and Department of Psychiatry, McGill University, Montreal, QC, Canada.

Phyllis Zelkowitz (P)

Jewish General Hospital, Lady Davis Institute for Medical Research and Department of Psychiatry, McGill University, Montreal, QC, Canada. phyllis.zelkowitz@mcgill.ca.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Méthylation de l'ADN › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › ADN intergénique › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Système stomatognathique › Bouche › Muqueuse de la bouche › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones hypophysaires › Hormones post-hypophysaires › Ocytocine › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur hormone hypophysaire › Récepteurs à l'ocytocine › Evaluating Methylation of the Oxytocin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Neuropeptides › Vasopressines › Evaluating Methylation of the Oxytocin...