Loss of haspin suppresses cancer cell proliferation by interfering with cell cycle progression at multiple stages.
Cell Cycle
/ drug effects
Cell Line, Tumor
Cell Proliferation
/ drug effects
Fluorescent Dyes
G1 Phase Cell Cycle Checkpoints
/ drug effects
G2 Phase
/ drug effects
Humans
Interphase
/ drug effects
Intracellular Signaling Peptides and Proteins
/ antagonists & inhibitors
Mitosis
/ drug effects
Neoplasms
/ genetics
Protein Serine-Threonine Kinases
/ antagonists & inhibitors
S Phase
/ drug effects
Tubercidin
/ analogs & derivatives
Tumor Suppressor Protein p53
/ genetics
Ubiquitination
Up-Regulation
/ drug effects
cell cycle
haspin
kinase
mitosis
p21
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
18
08
2021
received:
16
01
2021
accepted:
31
08
2021
entrez:
22
9
2021
pubmed:
23
9
2021
medline:
28
10
2021
Statut:
ppublish
Résumé
Our recent studies have shown that haspin, a protein kinase imperative for mitosis, is engaged in the interphase progression of HeLa and U2OS cancer cells. In this investigation, we employed the Fucci reporter system and time-lapse imaging to examine the impact of haspin gene silencing on cell cycle progressions at a single-cell level. We found that the loss of haspin induced multiple cell cycle defects. Specifically, the S/G2 duration was greatly prolonged by haspin gene depletion or inhibition in synchronous HeLa cells. Haspin gene depletion in asynchronous HeLa and U2OS cells led to a similarly protracted S/G2 phase, followed by mitotic cell death or postmitotic G1 arrest. In addition, haspin deficiency resulted in robust induction of the p21
Identifiants
pubmed: 34551143
doi: 10.1096/fj.202100099R
doi:
Substances chimiques
Fluorescent Dyes
0
Intracellular Signaling Peptides and Proteins
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
5-iodotubercidin
24386-93-4
HASPIN protein, human
EC 2.7.11.1
Protein Serine-Threonine Kinases
EC 2.7.11.1
Tubercidin
M351LCX45Y
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21923Informations de copyright
© 2021 Federation of American Societies for Experimental Biology.
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