Evolution of Cytomegalovirus-Responsive T Cell Clonality following Solid Organ Transplantation.
Adult
Aged
Antigens, Viral
/ immunology
CD4-Positive T-Lymphocytes
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Cell Proliferation
Clone Cells
Cytomegalovirus
/ physiology
Cytomegalovirus Infections
/ immunology
Female
Genetic Variation
Graft Rejection
/ immunology
Heart Transplantation
Humans
Immunologic Memory
Kidney Transplantation
Male
Middle Aged
Receptors, Antigen, T-Cell, alpha-beta
/ genetics
Transplantation, Homologous
Virus Activation
/ immunology
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 10 2021
15 10 2021
Historique:
received:
29
04
2021
accepted:
04
08
2021
pubmed:
24
9
2021
medline:
16
11
2021
entrez:
23
9
2021
Statut:
ppublish
Résumé
CMV infection is a significant complication after solid organ transplantation. We used single cell TCR αβ sequencing to determine how memory inflation impacts clonality and diversity of the CMV-responsive CD8 and CD4 T cell repertoire in the first year after transplantation in human subjects. We observed CD8 T cell inflation but no changes in clonal diversity, indicating homeostatic stability in clones. In contrast, the CD4 repertoire was diverse and stable over time, with no evidence of CMV-responsive CD4 T cell expansion. We identified shared CDR3 TCR motifs among patients but no public CMV-specific TCRs. Temporal changes in clonality in response to transplantation and in the absence of detectable viral reactivation suggest changes in the repertoire immediately after transplantation followed by an expansion with stable clonal competition that may mediate protection.
Identifiants
pubmed: 34551964
pii: jimmunol.2100404
doi: 10.4049/jimmunol.2100404
pmc: PMC8492537
mid: NIHMS1733317
doi:
Substances chimiques
Antigens, Viral
0
Receptors, Antigen, T-Cell, alpha-beta
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2077-2085Subventions
Organisme : CSRD VA
ID : I01 CX001971
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK116074
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125197
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007290
Pays : United States
Organisme : NIDDK NIH HHS
ID : K01 DK123196
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI057229
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007357
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI109662
Pays : United States
Informations de copyright
Copyright © 2021 by The American Association of Immunologists, Inc.
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