Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles.


Journal

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
ISSN: 1537-6591
Titre abrégé: Clin Infect Dis
Pays: United States
ID NLM: 9203213

Informations de publication

Date de publication:
30 05 2022
Historique:
received: 05 07 2021
pubmed: 24 9 2021
medline: 3 6 2022
entrez: 23 9 2021
Statut: ppublish

Résumé

We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

Identifiants

pubmed: 34554228
pii: 6374445
doi: 10.1093/cid/ciab848
doi:

Substances chimiques

Bacterial Proteins 0
beta-Lactamases EC 3.5.2.6
carbapenemase EC 3.5.2.6

Banques de données

ClinicalTrials.gov
['NCT02743585']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1850-1854

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Auteurs

Sean Wei Xiang Ong (SWX)

National Centre for Infectious Diseases, Singapore.
Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.

Pei Yun Hon (PY)

National Centre for Infectious Diseases, Singapore.

Sharon Syn Hui Wee (SSH)

Clinical Research and Innovation Office, Tan Tock Seng Hospital, Singapore.

Jonathan Wei Zhong Chia (JWZ)

Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore.

Shehara Mendis (S)

Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore.

Ezlyn Izharuddin (E)

National Centre for Infectious Diseases, Singapore.

Ray Junhao Lin (RJ)

National Centre for Infectious Diseases, Singapore.
Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.

Po Ying Chia (PY)

National Centre for Infectious Diseases, Singapore.
Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.

Rees Chin Swee Sim (RCS)

Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore.

Mark I-Cheng Chen (MI)

National Centre for Infectious Diseases, Singapore.
Saw Swee Hock School of Public Health, National University of Singapore, Singapore.

Angela Chow (A)

Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
Saw Swee Hock School of Public Health, National University of Singapore, Singapore.
Department of Clinical Epidemiology, Office of Clinical Epidemiology, Analytics, and Knowledge (OCEAN), Tan Tock Seng Hospital, Singapore.

Joanne Yoong (J)

Saw Swee Hock School of Public Health, National University of Singapore, Singapore.

David Chien Lye (DC)

National Centre for Infectious Diseases, Singapore.
Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Christine B Teng (CB)

Department of Pharmacy, National University of Singapore, Singapore.

Paul Anantharajah Tambyah (PA)

Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Division of Infectious Disease, National University Hospital, Singapore.

Ritu Banerjee (R)

Division of Pediatric Infectious Diseases, Vanderbilt University, Nashville, Tennessee, USA.

Robin Patel (R)

Divisions of Clinical Microbiology and Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA.

Partha Pratim De (PP)

Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore.

Shawn Vasoo (S)

National Centre for Infectious Diseases, Singapore.
Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.

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Classifications MeSH