Rapeseed Transformation with aroA Bacterial Gene Containing P101S Mutation Confers Glyphosate Resistance.
Aromatic acid
Brassica napus
Mutant gene
Roundup
Shikimic pathway
Journal
Biochemical genetics
ISSN: 1573-4927
Titre abrégé: Biochem Genet
Pays: United States
ID NLM: 0126611
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
29
07
2020
accepted:
16
09
2021
pubmed:
25
9
2021
medline:
7
5
2022
entrez:
24
9
2021
Statut:
ppublish
Résumé
Field weed infestations can cause serious problems and require regular and planned programs to control them. Glyphosate is a broad-spectrum herbicide that inactivates the 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) enzyme and causes plant death. It has been reported that the mutation of proline 101 to serine in EPSPS is one of the effective mutations to reduce the affinity of glyphosate to EPSPS enzyme. In this study, we investigated the effect of the bacterial P101S mutant aromatic acid (aroA) gene on glyphosate resistance in transgenic rapeseeds. For this purpose, the mutant gene was synthesized and cloned into the pUC18 and pBI121 vectors. The gene was transferred to rapeseed by the Agrobacterium-mediated method. In this experiment, three generations of transgenic plants (T0, T1, and T2) were studied under in vitro and in vivo conditions. After the treatment of 75 putative transgenic plants with 10 mM glyphosate in T0 generation, resistant plants were identified and their seeds were harvested. In the T1 generation, out of 200 cultivated plants, 141 showed resistance. After the plants were treated with herbicides and resistance was determined, the seeds were harvested when they mature. In the T2 generation, most plants (162 plants of 200) were resistant to glyphosate. Therefore, the inheritance of resistance followed Mendel's first law, which is a sign of the monogenic character of resistance. Purification and increasing the percentage of resistant plants will be carried out in the next generations. It is concluded that P101S mutation guarantees glyphosate resistance of rapeseed and is recommended to study it in other plants.
Identifiants
pubmed: 34559349
doi: 10.1007/s10528-021-10136-w
pii: 10.1007/s10528-021-10136-w
doi:
Substances chimiques
Herbicides
0
3-Phosphoshikimate 1-Carboxyvinyltransferase
EC 2.5.1.19
Glycine
TE7660XO1C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
953-968Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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