Combining stretching and gallic acid to decrease inflammation indices and promote extracellular matrix production in osteoarthritic human articular chondrocytes.
Cartilage, Articular
/ drug effects
Cells, Cultured
Chondrocytes
/ drug effects
Collagen Type I, alpha 1 Chain
/ genetics
Collagen Type II
/ genetics
Collagen Type X
/ genetics
Collagen Type XI
/ genetics
Extracellular Matrix
/ drug effects
Gallic Acid
/ pharmacology
Gene Expression Regulation
/ drug effects
Humans
Inflammation
/ genetics
Matrix Metalloproteinase 1
/ genetics
Matrix Metalloproteinase 13
/ genetics
Muscle Stretching Exercises
Osteoarthritis
/ genetics
And inflammation
Articular cartilage tissue engineering
Chondrocytes
Collagen
Cyclic tensile stress
Gallic acid
Glycosaminoglycan
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
15 11 2021
15 11 2021
Historique:
received:
02
03
2021
revised:
21
08
2021
accepted:
22
09
2021
pubmed:
27
9
2021
medline:
15
12
2021
entrez:
26
9
2021
Statut:
ppublish
Résumé
Osteoarthritis (OA) patients undergo cartilage degradation and experience painful joint swelling. OA symptoms are caused by inflammatory molecules and the upregulation of catabolic genes leading to the breakdown of cartilage extracellular matrix (ECM). Here, we investigate the effects of gallic acid (GA) and mechanical stretching on the expression of anabolic and catabolic genes and restoring ECM production by osteoarthritic human articular chondrocytes (hAChs) cultured in monolayers. hAChs were seeded onto conventional plates or silicone chambers with or without 100 μM GA. A 5% cyclic tensile strain (CTS) was applied to the silicone chambers and the deposition of collagen and glycosaminoglycan, and gene expressions of collagen types II (COL2A1), XI (COL11A2), I (COL1A1), and X (COL10A1), and matrix metalloproteinases (MMP-1 and MMP-13) as inflammation markers, were quantified. CTS and GA acted synergistically to promote the deposition of collagen and glycosaminoglycan in the ECM by 14- and 7-fold, respectively. Furthermore, the synergistic stimuli selectively upregulated the expression of cartilage-specific proteins, COL11A2 by 7-fold, and COL2A1 by 47-fold, and, in contrast, downregulated the expression of MMP-1 by 2.5-fold and MMP-13 by 125-fold. GA supplementation with CTS is a promising approach for restoring osteoarthritic hAChs ECM production ability making them suitable for complex tissue engineering applications.
Identifiants
pubmed: 34563516
pii: S0014-4827(21)00395-5
doi: 10.1016/j.yexcr.2021.112841
pmc: PMC9495269
mid: NIHMS1835470
pii:
doi:
Substances chimiques
COL10A1 protein, human
0
COL11A2 protein, human
0
COL2A1 protein, human
0
Collagen Type I, alpha 1 Chain
0
Collagen Type II
0
Collagen Type X
0
Collagen Type XI
0
Gallic Acid
632XD903SP
MMP13 protein, human
EC 3.4.24.-
Matrix Metalloproteinase 13
EC 3.4.24.-
MMP1 protein, human
EC 3.4.24.7
Matrix Metalloproteinase 1
EC 3.4.24.7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
112841Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008336
Pays : United States
Informations de copyright
Copyright © 2021. Published by Elsevier Inc.
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