Engineering an Antibody V Gene-Selective Vaccine.
Adoptive Transfer
Animals
Antibody Specificity
B-Lymphocytes
/ drug effects
Female
Gene Library
Humans
Ligands
Male
Mice, Transgenic
Proof of Concept Study
Protein Engineering
RNA Phages
/ genetics
Receptors, Antigen, B-Cell
/ genetics
Single-Domain Antibodies
/ administration & dosage
Vaccination
Vaccines, Virus-Like Particle
/ administration & dosage
B cell receptor
VLP
antibody
lineage
prime
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
25
06
2021
accepted:
25
08
2021
entrez:
27
9
2021
pubmed:
28
9
2021
medline:
24
12
2021
Statut:
epublish
Résumé
The ligand-binding surface of the B cell receptor (BCR) is formed by encoded and non-encoded antigen complementarity determining regions (CDRs). Genetically reproducible or 'public' antibodies can arise when the encoded CDRs play deterministic roles in antigen recognition, notably within human broadly neutralizing antibodies against HIV and influenza virus. We sought to exploit this by engineering virus-like-particle (VLP) vaccines that harbor multivalent affinity against gene-encoded moieties of the BCR antigen binding site. As proof of concept, we deployed a library of RNA bacteriophage VLPs displaying random peptides to identify a multivalent antigen that selectively triggered germline BCRs using the human V
Identifiants
pubmed: 34566992
doi: 10.3389/fimmu.2021.730471
pmc: PMC8459710
doi:
Substances chimiques
Ligands
0
Receptors, Antigen, B-Cell
0
Single-Domain Antibodies
0
Vaccines, Virus-Like Particle
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
730471Subventions
Organisme : NIAID NIH HHS
ID : R01 AI153098
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI155447
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI057229
Pays : United States
Organisme : NIDA NIH HHS
ID : DP2 DA042422
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007538
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124378
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI137057
Pays : United States
Informations de copyright
Copyright © 2021 Ronsard, Yousif, Peabody, Okonkwo, Devant, Mogus, Barnes, Rohrer, Lonberg, Peabody, Chackerian and Lingwood.
Déclaration de conflit d'intérêts
BC and DP have an equity stake in Flagship Laboratories 72. RMB, DR and NL were employed by Bristol Myers Squibb. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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