Protein Corona Inhibits Endosomal Escape of Functionalized DNA Nanostructures in Living Cells.
DNA nanotechnology
bionano interactions
cellular uptake
endolysosomal escape
nanotechnology
protein corona
Journal
ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991
Informations de publication
Date de publication:
06 Oct 2021
06 Oct 2021
Historique:
pubmed:
28
9
2021
medline:
1
2
2022
entrez:
27
9
2021
Statut:
ppublish
Résumé
DNA nanostructures (DNs) can be designed in a controlled and programmable manner, and these structures are increasingly used in a variety of biomedical applications, such as the delivery of therapeutic agents. When exposed to biological liquids, most nanomaterials become covered by a protein corona, which in turn modulates their cellular uptake and the biological response they elicit. However, the interplay between living cells and designed DNs are still not well established. Namely, there are very limited studies that assess protein corona impact on DN biological activity. Here, we analyzed the uptake of functionalized DNs in three distinct hepatic cell lines. Our analysis indicates that cellular uptake is linearly dependent on the cell size. Further, we show that the protein corona determines the endolysosomal vesicle escape efficiency of DNs coated with an endosome escape peptide. Our study offers an important basis for future optimization of DNs as delivery systems for various biomedical applications.
Identifiants
pubmed: 34569777
doi: 10.1021/acsami.1c14401
pmc: PMC9590277
mid: NIHMS1839826
doi:
Substances chimiques
Antimicrobial Cationic Peptides
0
Protein Corona
0
aurein 1.2 peptide
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
46375-46390Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM132931
Pays : United States
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