Impact of DICER1 and DROSHA on the Angiogenic Capacity of Human Endothelial Cells.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
12 Sep 2021
Historique:
received: 20 08 2021
revised: 08 09 2021
accepted: 10 09 2021
entrez: 28 9 2021
pubmed: 29 9 2021
medline: 25 2 2023
Statut: epublish

Résumé

RNAi-mediated knockdown of DICER1 and DROSHA, enzymes critically involved in miRNA biogenesis, has been postulated to affect the homeostasis and the angiogenic capacity of human endothelial cells. To re-evaluate this issue, we reduced the expression of DICER1 or DROSHA by RNAi-mediated knockdown and subsequently investigated the effect of these interventions on the angiogenic capacity of human umbilical vein endothelial cells (HUVEC)

Identifiants

pubmed: 34576018
pii: ijms22189855
doi: 10.3390/ijms22189855
pmc: PMC8471234
pii:
doi:

Substances chimiques

DICER1 protein, human EC 3.1.26.3
DROSHA protein, human EC 3.1.26.3
Ribonuclease III EC 3.1.26.3
DEAD-box RNA Helicases EC 3.6.4.13

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : European Regional Development Fund of the European Commission
ID : W21029490
Organisme : Publication Fund of the Martin-Luther-University Halle/Wittenberg
ID : VAT DE 811353703

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Auteurs

Heike Braun (H)

Department of Clinical Pharmacy and Pharmacotherapy, Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Michael Hauke (M)

Department of Clinical Pharmacy and Pharmacotherapy, Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Anne Ripperger (A)

Department of Clinical Pharmacy and Pharmacotherapy, Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Christian Ihling (C)

Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Charles Tanford Center, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Matthew Fuszard (M)

Core Facility-Proteomics Mass Spectrometry, Charles Tanford Centre, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Robert Eckenstaler (R)

Department of Clinical Pharmacy and Pharmacotherapy, Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.

Ralf A Benndorf (RA)

Department of Clinical Pharmacy and Pharmacotherapy, Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, 06120 Halle (Saale), Germany.

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Classifications MeSH